BRCA promoter methylation in sporadic versus BRCA germline mutation-related breast cancers

Abstract

Background

In breast cancer, BRCA promoter hypermethylation and BRCA germline mutations are said to occur together rarely, but this property has not yet been translated into a clinical test. Our aim in this study was to investigate the diagnostic value of BRCA1/2 methylation in distinguishing breast carcinomas of BRCA1 and BRCA2 germline mutation carriers from sporadic breast carcinomas using a recently developed BRCA methylation assay based on methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA).

Methods

MS-MLPAs were performed to assess BRCA1 and BRCA2 methylation in breast carcinoma tissues from 39 BRCA1 and 33 BRCA2 germline mutation carriers, 80 patients with sporadic breast cancer, and normal breast tissues from 5 BRCA1 and 4 BRCA2 mutation carriers and 5 nonmutation carriers.

Results

Methylation frequencies varied considerably between CpG sites across the BRCA1 and BRCA2 promoters. Some CpG sites were methylated more frequently in BRCA1/2-related than in sporadic carcinomas, whereas other CpG sites were methylated more frequently in sporadic carcinomas, with large variances in sensitivity and specificity as a consequence.

Conclusions

The diagnostic value of BRCA promoter methylation analysis in distinguishing BRCA1/2-related from sporadic breast carcinomas seems to be considerably dependent on the targeted CpG sites. These findings are important for adequate use of BRCA methylation analysis as a prescreening tool for BRCA germline genetic testing or to identify BRCAness patients who may benefit from targeted therapies such as poly(adenosine diphosphate-ribose) polymerase inhibitors.

Background

Breast cancer is the most frequent cancer type in women worldwide [1]. In about 5–10%, breast cancer occurs in a hereditary setting, most commonly due to BRCA1 or BRCA2 germline mutations, which lead to a 40–80% lifetime risk of developing breast cancer as well as a 30–40% lifetime risk of ovarian cancer development [2,3,4,5,6,7,8]. Promoter hypermethylation plays an important role in carcinogenesis of several organs, including the breast, because hypermethylation of cytosine phosphate guanine (CpG) sites in promoter regions may lead to downregulation of tumor suppressor genes [9,10,11,12,13,14,15]. It has been proposed in the literature that BRCA promoter hypermethylation takes place almost exclusively in the sporadic setting and only rarely occurs in patients with an underlying BRCA1 or BRCA2 germline mutation [16,17,18,19,20,21,22,23,24,25,26]. This is potentially clinically important because promoter methylation assays could then serve as prescreening tests when a hereditary nature is suspected, obviating the need for germline mutation analysis in cases of promoter methylation. However, for routine testing, more confirmation is mandatory, such as with regard to the best CpG sites to target, and a robust assay needs to be at hand that works on small amounts of fragmented DNA from formalin-fixed, paraffin-embedded (FFPE) tumor material. The latter is also important in view of the growing need to test for BRCA1 and BRCA2 (BRCA1/2) promoter methylation as a sign of BRCAness, which may provide an indication for treatment with poly(adenosine diphosphate-ribose) polymerase (PARP) inhibitors [27,28,29,30,31]. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is a rapid, robust, and inexpensive multiplex methylation test that works well on small amounts of DNA derived from FFPE tissues. The aim of this study was to investigate the diagnostic value of BRCA1/2 promoter methylation in distinguishing breast carcinomas from BRCA1 and BRCA2 germline mutation carriers (BRCA1/2-related breast carcinomas) and sporadic breast carcinomas using a recently developed BRCA methylation MS-MLPA assay. In other words, we sought to determine to what extent BRCA1/2 promoter methylation can be detected in BRCA1/2-related compared with sporadic breast carcinomas.

Methods

Patient material

FFPE tissues of 39 BRCA1 and 33 BRCA2 germline mutation-related breast cancer resection specimens (BRCA1/2-C) were derived from the pathology archives at the University Medical Center Utrecht, University Medical Center Groningen, VU University Medical Center Amsterdam, and local hospitals around Utrecht, The Netherlands. Also, FFPE tissues of prophylactic mastectomy specimens of five BRCA1 and four BRCA2 germline mutation carriers (BRCA1/2-N) were derived from the pathology archives of the University Medical Center Utrecht. BRCA status had been confirmed through mutation analysis at a medical genetics department within The Netherlands after informed consent. For comparison, FFPE tissues of 80 breast cancer resection (Sporadic-C) and 5 breast reduction samples (non-BRCA-related-N) from women not tested for a BRCA mutation were derived from the pathology archive of the University Medical Center Utrecht. These women did not receive BRCA germline mutation testing, because there was no clinical suspicion of a hereditary nature. No further inclusion or exclusion criteria were applied. From the tissue blocks, 4-μm-thick sections were cut and stained with hematoxylin and eosin. Tumor characterization, grading according to the modified Bloom-Richardson grading system [32], and scoring of immunohistochemical staining were performed by an experienced breast pathologist (PJvD), who was blinded to mutation status. Estrogen receptor (ER) and progesterone receptor (PR) immunohistochemical staining was considered positive when ≥10% of the tumor cells showed expression, regardless of intensity. Human epidermal growth factor receptor 2 (HER2) was scored according to the HercepTest scoring system (Dako, Glostrup, Denmark) for breast cancer, where only a 3+ score was considered positive. The clinicopathological characteristics are provided in Table 1.

Table 1 Clinicopathological characteristics of included breast samples

DNA isolation

Normal breast and breast cancer tissues were harvested from 10×10-μm-thick and 4×4-μm-thick tissue sections, respectively. Areas with necrosis, preinvasive lesions, and extensive inflammation were avoided. DNA isolation was performed by overnight incubation at 56 °C in lysis buffer (50 mM Tris-HCl, pH 8.0, 0.5% Tween 20) with proteinase K (10 mg/ml; Roche, Basel, Switzerland). Proteinase K was deactivated by boiling for 10 minutes. After centrifugation for 2 minutes at 14,000 rpm, the supernatant was collected for further analysis. DNA content was measured using an ND-1000 spectrophotometer (NanoDrop Products, Wilmington, DE, USA).

Methylation analysis

Five microliters of supernatant with a DNA concentration between 50 and 500 ng/μl were used for MS-MLPA analysis according to the manufacturer’s instructions, using the ME053 BRCA1-BRCA2 X1-0914 methylation assay (MRC-Holland, Amsterdam, The Netherlands). When the DNA concentration exceeded 500 ng/μl, the input volume was adjusted proportionally. The ME053 methylation assay contains three and four probes to detect BRCA1 and BRCA2 promoter methylation, respectively, enabling methylation status determination of three CpG sites in the BRCA1 promoter region and five CpG sites in the BRCA2 promoter region (see Table 2 and Fig. 1 for further details). The MS-MLPA principle and analytical procedure are described elsewhere [33], and the technique has been shown to be reliable for methylation assessment [33,34,35,36,37]. Samples that were 100% methylated (SssI methyltransferase-treated MDA-MB-231 and A549 cells) were used as positive controls, and normal peripheral blood samples were used as negative controls. No template controls were included. Moreover, the methylation assay included two digestion (methylation) control probes.

Table 2 Methylation probe characteristics of the methylation-specific multiplex ligation-dependent probe amplification assay (ME053 BRCA1-BRCA2 X1-0914; MRC-Holland)

Fig. 1

Overview of the targeted cytosine phosphate guanine (CpG) sites in the BRCA1 and BRCA2 promoter regions by methylation analysis of the selected studies

Coffalyser.Net software (MRC-Holland) was used for methylation data analysis. Quality control showed that the results of the control probes and control samples were adequate. The methylation percentage cutoff per probe was set at the highest methylation percentage value in normal breast tissues from nonmutation carriers (non-BRCA-related N), ranging from >15% to >19% (see also Fig. 2). Moreover, the cumulative methylation index (CMI) was calculated as the sum of the methylation percentage of all methylation probes. MS-MLPA analysis was performed by SV and CBM, who were blinded to mutation status.

Fig. 2

a–g Distribution of absolute methylation percentages for all BRCA1 and BRCA2 methylation probes. BRCA1-C Breast carcinomas from BRCA1 germline mutation carriers, BRCA2-C Breast carcinomas from BRCA2 germline mutation carriers, BRCA1/2-N Normal breast tissue from BRCA1 and BRCA2 germline mutation carriers, Sporadic-C Sporadic breast carcinoma

Statistical analysis

Statistical analyses were performed using IBM SPSS Statistics version 23.0 software (IBM, Armonk, NY, USA). Associations between absolute methylation percentages, CMI or age, and mutation status (BRCA1/2-related carcinomas versus sporadic carcinomas) were assessed by the Mann-Whitney U test. Associations between dichotomized BRCA promoter methylation and mutation status or other clinicopathological characteristics were assessed by Pearson’s chi-square test or Fisher’s exact test. Sensitivity and specificity were calculated. Correlations between CMI and age were assessed using the Spearman’s rho correlation coefficient. The level of significance used was set at a two-sided p value <0.05.

Correlation between BRCA1/2 methylation and messenger RNA expression

The Wanderer tool was used to assess the correlation between BRCA1/2 methylation and messenger RNA (mRNA) expression. This tool was created on the basis of data from The Cancer Genome Atlas (TCGA) Research Network [38]. The Infinium 450K HumanMethylation Array (Illumina, San Diego, CA, USA) was selected as the methylation data type, and Spearman’s correlation coefficient was selected as the correlation method.

Results

BRCA1 promoter methylation in BRCA1/2-related and sporadic breast carcinomas

The absolute methylation percentages and their distribution varied considerably between the three BRCA1 methylation probes (Table 3, Fig. 2). For the BRCA1.2 and BRCA1.3 probes, BRCA1/2-C showed significantly higher median methylation percentages than Sporadic-C (p = 0.00006 and p = 0.00003, respectively). The dichotomized results are shown in Table 4. BRCA1/2-C showed significantly less frequent methylation with the BRCA1.1 probe (p = 0.019), but significantly more frequent methylation with the BRCA1.2 probe (p = 0.000009). Methylation of at least one of the three BRCA1 methylation probes was seen in 46 (63.9%) of 72 BRCA1/2-C compared with 22 (27.5%) of 80 Sporadic-C (p = 0.000009). The sensitivity and specificity of the BRCA1 methylation probes in distinguishing BRCA1/2-C from Sporadic-C are shown in Table 5. The calculation of the sensitivity and specificity differs between probes owing to differences in methylation frequencies between BRCA1/2-C and Sporadic-C (see Table 4 and explanation in Table 5). The BRCA1.1 and BRCA1.3 probes showed good performance in ruling out BRCA1/2 germline mutations when methylation was detected (sensitivity 97.2% and 90.3%, respectively), although the specificity was poor because many Sporadic-C did not show methylation with these probes either (specificity for both 13.8%). The BRCA1.2 probe and the combination of the three BRCA1 probes (BRCA1 total) showed moderate sensitivity (both 63.9%) and specificity (72.5%) when used to rule in BRCA1/2 germline mutations when methylation was present.

Table 3 BRCA promoter methylation percentages in BRCA1/2-related and sporadic breast carcinomas by methylation-specific multiplex ligation-dependent probe amplification

Table 4 Frequency of BRCA methylation (dichotomized results) in BRCA1/2-related and sporadic breast carcinomas by methylation-specific multiplex ligation-dependent probe amplification

Table 5 Sensitivity and specificity for each methylation probe in distinguishing BRCA1/2-related from sporadic breast carcinomas

To evaluate the robustness of the MS-MLPA assay, we compared the results of the ME053 assay with another MS-MLPA assay tested on Sporadic-C in our laboratory, the ME001 assay (MRC-Holland) (C. B. Moelans, unpublished observations; data not shown). One of the BRCA1 methylation probes in this assay determines the methylation status of the same CpG site as the BRCA1.3 probe in the ME053 MS-MLPA assay. There was a strong correlation in dichotomized BRCA1 promoter methylation results in Sporadic-C between the two assays (Spearman’s rho correlation coefficient, 0.831; p = 1000°10⁻¹³). For absolute methylation percentages, the correlation was weaker but still significant (Spearman’s rho correlation coefficient, 0.379; p = 0.001). In general, the Sporadic-C showed slightly higher BRCA1 methylation percentages with the ME001 assay. In 4 of 80 cases, BRCA1 was methylated according to the ME001 assay but unmethylated according to the ME053 assay. However, methylation percentages in these cases were only slightly above the threshold of 15% (17–20%) with the ME001 assay.

BRCA2 promoter methylation in BRCA1/2-related and sporadic breast carcinomas

BRCA1/2-C showed significantly higher median methylation percentages for all BRCA2 methylation probes than Sporadic-C, although the absolute methylation percentages and their distribution varied considerably between the four BRCA2 methylation probes (Table 3, Fig. 2). Using dichotomized results, BRCA1/2-C showed significantly more frequent methylation in all four probes, as shown in Table 4. When the dichotomized results of the BRCA2 methylation probes were combined, 50 (69.4%) of 72 BRCA1/2-C showed methylation of at least one of the four BRCA2 methylation probes, compared with 10 (12.5%) of 80 Sporadic-C (p = 0.029). The sensitivity and specificity of the BRCA1 methylation probes in distinguishing BRCA1/2-C from Sporadic-C are shown in Table 5. The BRCA2.2 and BRCA2.4 probes showed excellent specificity (both 100%) when used to rule in BRCA1/2 germline mutations when methylation was detected because no Sporadic-C were methylated with these probes. However, the sensitivity was poor (both 9.7%) because few BRCA1/2-C showed methylation. The BRCA2.1 and BRCA2.3, as well as the combination of all four BRCA2 probes (BRCA2 total), showed moderate sensitivity (50.0–69.4%) and rather good specificity (87.5–97.5%) when used to rule in BRCA1/2 germline mutations when methylation was detected.

Correlation with clinicopathological variables

As shown in Table 1, BRCA1/2-C and Sporadic-C differed significantly with respect to age, grade, and ER and PR status. We analyzed whether the differences we observed in methylation frequencies between BRCA1/2-C and Sporadic-C may be related to these differences in clinicopathological variables (Tables 6, 7 and 8). In Sporadic-C, methylation of the BRCA1.1, BRCA1.2, and BRCA1.3 probes, separately as well as combined (BRCA1 total), was significantly more frequently detected in grade 3 tumors than in grades 1–2 tumors and in ER-negative than in ER-positive tumors. Methylation of the BRCA1.1 and BRCA1.3 probes was also significantly more frequently detected in PR-negative tumors. For BRCA2 methylation in Sporadic-C, there was a statistically significant association only with grade: Methylation of the BRCA2.1 probe and of all four BRCA2 probes combined was more frequently seen in grade 3 carcinomas. There were no statistically significant correlations between BRCA1 and BRCA2 methylation on the one hand and between tumor type (ductal versus lobular carcinomas) and HER2 status on the other hand. In BRCA1-C and BRCA2-C, there were no statistically significant associations between BRCA1 or BRCA2 methylation and clinicopathological variables. Moreover, no statistically significant correlation was found between CMI for BRCA1 and/or BRCA2 promoter methylation and age in BRCA1-C, BRCA2-C, Sporadic-C, BRCA1/2-N, and non-BRCA-related-N (Table 9).

Table 6 Relationship between BRCA1/2 methylation and clinicopathological variables in sporadic breast carcinomas

Table 7 Relationship between BRCA1/2 methylation and clinicopathological variables in BRCA1-related carcinomas

Table 8 Relationship between BRCA1/2 methylation and clinicopathological variables in BRCA2-related carcinomas

Table 9 Correlation between age and cumulative methylation index for BRCA1/2 methylation

Correlation between BRCA1/2 methylation and mRNA expression

Methylation of the evaluated CpG sites within the BRCA1 and BRCA2 promoters showed weak correlations with mRNA levels by TCGA data extraction through the Wanderer viewer. The Spearman’s correlation coefficients between BRCA1 methylation and mRNA expression were −0.203 for cg04110421 (targeted by the BRCA1.1 probe), −0.296 for cg16630982 (targeted by the BRCA1.2 probe), and −0.172 for cg08993267 (targeted by the BRCA1.3 probe). For BRCA2, the CpG loci identifiers from the TCGA data most closely located to our MS-MLPA targets were used. Therefore, the correlation between BRCA2 methylation and mRNA expression should be interpreted with caution. The Spearman’s correlation coefficients between BRCA2 methylation and mRNA expression were −0.014 for cg20073910 (82 and 69 bp from CpG sites targeted by the BRCA2.1 and BRCA2.4 probes, respectively), 0.067 for cg27253386 (80 and 69 bp from the CpG sites targeted by the BRCA2.3 probe), and −0.092 for cg08157964 (25 bp from the CpG site targeted by the BRCA2.2 probe).

BRCA promoter methylation in BRCA1/2-related and non-BRCA-related normal breast tissue

BRCA1/2-N samples showed statistically significant higher absolute methylation percentages for the BRCA2.3 and BRCA2.4 probes (p = 0.031 and p = 0.005, respectively) (Table 10, Fig. 2). There was a borderline significant trend of higher methylation percentages for the BRCA1.2, BRCA1.3, and BRCA2.1 probes in BRCA1/2-N samples than for the non-BRCA-related-N cases (Table 10, Fig. 2). If methylation cutoffs per probe were based upon the highest methylation percentage found in non-BRCA-related-N cases, 40% (two of five) and 60% (three of five) of BRCA1-N cases would have at least one methylated BRCA1 probe and one methylated BRCA2 probe, respectively. BRCA2-N cases would have methylation of at least one BRCA1 probe and one BRCA2 probe in 25% (one of four) and 50% (two of four) of cases, respectively (Table 11).

Table 10 BRCA promoter methylation percentages in normal breast from BRCA1/2 germline mutation carriers and nonmutation carriers

Table 11 Frequency of BRCA methylation (dichotomized) in prophylactic mastectomies of BRCA1/2 germline mutation carriers by methylation-specific multiplex ligation-dependent probe amplification

Discussion

The aim of this study was to investigate the diagnostic value of BRCA1/2 promoter methylation analysis using a new BRCA methylation MS-MLPA assay in distinguishing sporadic breast carcinomas from BRCA1 and BRCA2 germline mutation-related carcinomas in order to arrive at a clinically applicable prescreening test for BRCA1/2-related cancers. We observed considerably varying frequencies of BRCA promoter methylation between the targeted CpG sites across the BRCA1 and BRCA2 promoters. Some CpG sites were methylated more frequently in BRCA1/2-C than in Sporadic-C (those targeted by the BRCA1.2, BRCA2.1, BRCA2.2, BRCA2.3, and BRCA2.4 probes), whereas other CpG sites were methylated more frequently in Sporadic-C (those targeted by the BRCA1.1 and BRCA1.3 probes). In general, we observed frequent BRCA promoter methylation in BRCA1/2-C. At least 63.8% (46 of 72) of BRCA1/2-C and 12.5% (10 of 80) of Sporadic-C showed methylation of at least one of the targeted CpG sites in the BRCA1 or BRCA2 promoter. Interestingly, several BRCA1-C showed BRCA2 promoter methylation and vice versa. Sensitivity and specificity varied considerably between the probes. The best probes for ruling out Sporadic-C when methylation was detected were BRCA2.2 and BRCA2.4 (specificity 100%). However, many BRCA1/2-C would be missed because the sensitivity was poor (9.7%). The best probes for ruling out BRCA1/2-C when methylation was not detected were BRCA1.1 and BRCA1.3 (sensitivity 97.2% and 90.3%, respectively). However, many Sporadic-C would be misclassified as potentially BRCA1/2 germline mutation-related because the specificity was poor (both 13.8%). Sensitivity and specificity were most balanced when all four BRCA2 probes were used to rule in BRCA1/2 germline mutations when methylation was detected in at least one the BRCA2 probes (sensitivity 69.4%, specificity 87.5%). BRCA1 promoter methylation was more frequent in high-grade, ER-negative, and PR-negative tumors. This finding is in line with other reports in the literature because BRCA1 methylation has been more frequently described in triple-negative breast carcinomas [39, 40]. BRCA2 promoter methylation was more frequent in high-grade tumors but showed no other statistically significant clinicopathological associations.

In line with our findings, Daniels et al. [41] recently demonstrated that DNA methylation levels vary between CpG sites in the BRCA1 promoter. However, our findings do not support the general assumption and previous findings reported in the literature that BRCA promoter methylation and BRCA germline mutations are mutually exclusive. In most studies, none of the BRCA-related breast carcinomas showed BRCA promoter methylation [16, 21, 23,24,25,26]. Kontorovich et al. [20] observed BRCA1 promoter methylation in 3 (6.3%) of 48 BRCA1-related breast carcinomas, and Tapia et al. [17] observed BRCA1 promoter methylation in 2 (66.7%) of 3 observed BRCA1-related breast carcinomas. Differences in observed methylation frequencies could be related to the technique and specific CpG sites targeted, the quality of input material, and the determination of methylation cutoffs in subsequent analysis. It should be noted that some patients with a BRCA germline mutation may develop breast cancer through sporadic breast carcinogenetic mechanisms, which could affect methylation frequencies.

Whether BRCA promoter methylation may occur as a second hit in BRCA1/2-related breast carcinomas is still unclear. The main question is whether methylation really drives carcinogenesis or whether it can be considered a bystander. Interestingly, in our study, normal breast tissues from BRCA1/2 germline mutation carriers showed BRCA2 promoter methylation levels compared with normal breast tissues from patients without BRCA germline mutations, although the sample size was limited. Bijron et al. [42] described increased BRCA2 promoter methylation in normal and precursor fallopian tube tissues from BRCA germline mutation carriers compared with normal sporadic fallopian tube tissues. BRCA methylation might therefore play a role in carcinogenesis in a subset of BRCA germline mutation carriers.

To our knowledge, our present study is the largest one to date investigating both BRCA1 and BRCA2 promoter methylation in BRCA1 as well as BRCA2 germline mutation-related breast carcinomas. Moreover, this is the first MS-MLPA study to specifically test BRCA promoter methylation in BRCA1- and BRCA2-related breast carcinomas compared with sporadic breast carcinomas, as well as the first MS-MLPA study in which BRCA methylation levels have been investigated in normal breast tissues of BRCA carriers. We validated our results for one of the BRCA methylation probes by comparing them with data obtained from a previous MS-MLPA experiment using the commercially available ME001 MS-MLPA assay.

Our findings may have important implications for clinical practice, such as prescreening for BRCA germline genetic testing or eligibility for certain therapeutic strategies. BRCA1 promoter methylation analysis has been proposed as a cost-effective and reliable prescreening tool to exclude BRCA1 germline mutations in patients with breast cancer similar to MLH1 promoter methylation and Lynch syndrome [22, 43]. Moreover, recent research shows that breast and ovarian carcinomas with BRCA deficiencies, including BRCA methylation, may also benefit from PARP inhibitor therapy [30, 31, 44,45,46,47,48,49].

Although MS-MLPA has been shown to be a reliable tool to assess methylation in general, it targets single specific sites targetable by the HhaI methylation-sensitive restriction enzyme. For MS-MLPA to be a reliable prescreening tool for ruling in or ruling out BRCA germline mutations and/or determining sensitivity for targeted therapy, a review of existing literature and further research, preferably assessing all CpG sites in the BRCA promoter regions (e.g., by methylation-specific polymerase chain reaction), is needed to determine the most predictive CpG sites for each indication. The most predictive CpG sites should then be targetable by the HhaI methylation-sensitive restriction enzyme because otherwise MS-MLPA may not be the preferred methylation analytical technique in this context.

Conclusions

The diagnostic value of BRCA promoter methylation analysis in distinguishing BRCA1/2-related and sporadic breast carcinomas is considerably dependent on the targeted CpG sites. These findings are important for adequate use of BRCA methylation analysis as a prescreening tool for germline genetic testing or to identify patients who may benefit from targeted therapies such as PARP inhibitors, making their way to the clinic for breast cancer. Further research is needed to assess which other CpG sites are important in ruling in or ruling out BRCA germline mutations or determining sensitivity for targeted therapy.