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Correction to: ECM1 regulates cell proliferation and trastuzumab resistance through activation of EGF-signaling

The original article was published in Breast Cancer Research 2014 16:479

Correction to: Breast Cancer Res

https://doi.org/10.1186/s13058-014-0479-6

After the publication of this work [1] errors were found in Figs. 1a and 5d. In the second column of Fig. 1a, an image from matrigel cultures of BT-474 wild type cells were mistakenly used for BT-474 TR shE cells. In Fig. 5d, the same blots were inserted for immunoprecipiation: HER3/Western blots: Mucin1 and immunoprecipitation Mucin1/Western blots: HER3. The corrected figures are shown below. We confirmed that the corrected figures do not affect conclusion and findings of the article. We sincerely apologize for the errors.

Fig. 1
figure1

ECM1 confers resistance toward Ttzm. a Cells were seeded with matrigel and treated with Ttzm (20 μg/mL) and rhECM1 (200 ng/mL). The numbers of colonies 20 μm or greater in diameter were counted at 12 days (*p < 0.05, **p < 0.005). b At 24 h after cell seeding, each cell line was treated with anti-ECM1 antibody (5 μg/mL) and Ttzm (20 μg/mL) in fresh medium. After a further 48 h, cell viability was analyzed using an MTT assay (*p < 0.05, **p < 0.005, ***p < 0.0005). c BT-474 vector and ECM1 cells and BT-474 TR control shRNA and ECM1 shRNA cells were passaged in the mice by subcutaneous injection into the lower flank of each mouse. When the tumor size increased up to 250 mm3, Ttzm at 20 mg/kg was administered to each mouse by i.p. injection twice per week (n = 5 or 6 for each group). d Circulating levels of ECM1 in serum from Ttzm-resistant breast cancer patients were assessed by ELISA (left) and Western blot analysis, and compared with (right) corresponding data for Ttzm-responsive patients (*p < 0.05)

Fig. 5
figure2

ECM1 stabilizes EGFR and HER3 proteins through galectin-3/MUC1. a Lysates from each cell line were analyzed by Western blotting. b At 24 h after seeding, BT-474 TR cells were transfected with each siRNA, incubated further for 48 h, and analyzed on Western blots. c At 24 h after seeding, cells were treated with rhECM1 (200 ng/mL) and incubated further for 48 h. Cell lysates were then incubated with MUC1, EGFR and HER3 antibodies overnight. Immunoprecipitates were analyzed on Western blots. d Total cell lysates were incubated with MUC1, EGFR and HER3 antibodies overnight, and immunoprecipitates were then analyzed on Western blots

Reference

  1. 1.

    Lee KM, Nam K, Oh S, Lim J, Kim YP, Lee JW, Yu JH, Ahn SH, Kim SB, Noh DY, Lee T, Shin I. ECM1 regulates cell proliferation and trastuzumab resistance through activation of EGF-signaling. Breast Cancer Res. 2014;16(6):479–95.

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Correspondence to Incheol Shin.

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