Volume 5 Supplement 1

24th Congress of the International Association for Breast Cancer Research. Advances in human breast cancer research: preclinical models

Open Access

Genetic manipulation of the mammary gland by transplantation

  • P Edwards1
Breast Cancer Research20035(Suppl 1):6

https://doi.org/10.1186/bcr665

Published: 1 October 2003

Mammary epithelium can be reconstituted in vivo by transplanting fragments of mammary epithelium, or suspensions of mammary epithelial cells, into the 'cleared' mammary fat pad of a syngeneic recipient mouse. A 'cleared' mammary fat pad is one from which the natural epithelium has been removed at 3 weeks of age. Genes can be introduced into the epithelium before transplantation using retrovirus vectors, or the epithelium can be taken from a knockout mouse [1]. The applications of transplantation, and its advantages and disadvantages compared with transgenesis will be surveyed, including the ability to use hormone-insensitive promoters; to introduce genes into clones of cells rather than whole tissues; the ease of studying early preneoplastic change; and the use of transplantation with transgenic knockouts, to rescue embryonic lethals and to distinguish systemic from local effects [2].

Authors’ Affiliations

(1)
Hutchison-MRC Research Centre, University of Cambridge

References

  1. Edwards , et al: J Mammary Gland Biol Neoplasia. 1996, 1: 75-90.View ArticlePubMedGoogle Scholar
  2. Fantl , et al: . Dev Biol. 1999, 212: 1-11. 10.1006/dbio.1999.9329.View ArticlePubMedGoogle Scholar

Copyright

© BioMed Central 2003

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