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Fig. 5 | Breast Cancer Research

Fig. 5

From: Integrin alpha-2 and beta-1 expression increases through multiple generations of the EDW01 patient-derived xenograft model of breast cancer—insight into their role in epithelial mesenchymal transition in vivo gained from an in vitro model system

Fig. 5

a Quantitative, real-time RT-PCR gene expression of various E-cadherin transcriptional repressor genes (TWIST1, SNAI1, SNAI2) and correlation with expression of the hypoxic indicator gene Carbonic Anhydrase 9 (CAIX) in RNA extracted from tumours in the ED03 and EDW01 PDX models across serial passages. Mean expression values shown, error bars are standard error from the mean; mRNA from 3 xenografts per passage was analysed. dCT in the figure represents delta CT, or change in CT values calculated by subtracting the raw CT of the gene of interest from the raw CT of the housekeeping gene RPL32. b (i) Pearson correlation statistics of data shown in a. Pearson’s correlation statistics of ITGB1 and ITGA2 correlated with (ii) CD24 or (iii) CD44 in the PDX models. c Carbonic Anhydrase 9 immunohistochemisty in the ED03 and EDW01 PDX models with passage (p) numbers shown in the vertical plane. Images shown are representative of the following numbers of xenografts within their respective PDX passage numbers: ED03—p3: 6; p7: 7; p9: 1; p10: 1, p11: 1; EDW01—p3: 8; p4: 13; p5: 8; p6: 7; p7: 3. p value shown is two-tailed, and p < 0.05 defined as statistically significant and shaded grey in b. Scale bar, 50 μm

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Breast Cancer Research

ISSN: 1465-542X

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