Fig. 4
From: Obesity promotes the expansion of metastasis-initiating cells in breast cancer
Microenvironmental effects on tumor cells. Tumors from mice fed a high-fat diet (HFD) contain greater numbers of neutrophils (a, n = 6 regular diet (RD), n = 14 HFD). Clodronate liposomes treatment increases tumor weight (b, n = 5 RD, n = 4 HFD). Cross-transplantation experiments reveal that the effects of the obese microenvironment on tumor cells are permanent (c, n = 14). Immunofluorescent staining of tumors show increased epithelial-mesenchymal transition (EMT) features in HFD groups (d). Tumor cells in tumors from mice fed RD or HFD injected intravenously into RD mice show different metastasis initiating potential (e, n = 19). Clodro, clodronate liposomes; αSMA, alpha smooth muscle actin; l, lean; O, obese