Fig. 2

Obesity and not a high-fat diet (HFD) is responsible for tumor progression. FVB/N mice do not gain weight after 13 weeks of HFD (a, n = 4 regular diet (RD) and n = 5 HFD). Py230 tumors in C57BL/6 mice grow significantly faster in obese mice (b, n = 40), while in FVB/N mice PyMT tumors do not differ in growth dynamics between the RD and HFD groups (c, n = 4). C57BL/6 Rag2−/− mice do not gain weight on a HFD (d, n = 4 RD and n = 5 HFD), nor do Py230 tumors differ significantly in their growth dynamics when grafted in C57BL/6 Rag2−/− mice (e, n = 4 RD and n = 5 HFD). Immunohistochemical analysis of CD11b in the adipose tissue of RD and HFD C57BL/6 mice (scalebar 50 um) and quantification of crown-like structures in C57BL/6, FVB/N and C57BL/6 Rag2−/− mice (f). Quantitative PCR analysis of indicated targets in the adipose tissue of RD and HFD mice (g). Error bars in panels b, c and e indicate SEM. Ct, cycle threshold; Arbp, acidic ribosomal phosphoprotein P0