Open Access

Erratum to: Cancer progression by breast tumors with Pit-1-overexpression is blocked by inhibition of metalloproteinase (MMP)-13

  • Juan Sendon-Lago1,
  • Samuel Seoane1,
  • Noemi Eiro2,
  • Maria A. Bermudez1,
  • Manuel Macia3,
  • Tomas Garcia-Caballero4,
  • Francisco J. Vizoso2 and
  • Roman Perez-Fernandez1Email author
Breast Cancer Research201719:38

Received: 6 March 2017

Accepted: 6 March 2017

Published: 28 March 2017

The original article was published in Breast Cancer Research 2014 16:505


After the publication of this work [1] an error was noticed in Fig. 4d and 4f. In the migration and invasion assays the same image was used accidentally for the Pit-1 + shMMP-1 and Pit-1 + shMMP-13. The corrected figure is shown below. The error does not affect the findings or conclusion of the article. We apologize for this error.
Fig. 4

MMP-1 and MMP-13 knockdown reduces invasive features in MCF-7 cells with Pit-1 overexpression, and in MDA-MB-231 cells. a-b Wound-healing assay in (a) MCF-7 cells with Pit-1 overexpression (pRSV-hPit-1), and knockdown of MMP-1 (shMMP-1(1) and shMMP-1(2)) and MMP-13 (shMMP-13(1) and shMMP-13(2)); (B) MDA-MB-231 cells with knockdown of Pit-1 (siPit-1), MMP-1 (shMMP-1(1) and shMMP-1(2)), and MMP-13 (shMMP-13(1) and shMMP-13(2)). Distance between the wound edges was measured at 48 hours in three different assays, and data are represented as mean ± SD; ns = not significant. c-d Cell motility through uncoated filters (migration) at 24 hours in control MCF-7 cells (pRcRSV), Pit-1-overexpressing MCF-7 cells (pRSV-hPit-1), and Pit-1-overexpressing and knockdown of MMP-1 or MMP-13 MCF-7 cells (pRSV-hPit-1 + shMMP-1 or −13). e-f Cell motility through matrigel-coated filters at 48 hours in control cells, cells transfected with the pRSV-hPit-1 vector, and cells transfected with pRSV-hPit-1 and knockdown of MMP-1 (Pit-1 + shMMP-1) or MMP-13 (Pit-1 + shMMP-13). Numbers represent mean ± SD. Scale bar: 100 μm. MMP-1, matrix metalloproteinase-1; MMP-13, matrix metalloproteinase-13; Pit-1, POU class 1 homeobox 1



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Authors’ Affiliations

Department of Physiology- Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), School of Medicine, University of Santiago de Compostela
Unidad de Investigación, Fundacion Hospital de Jove
Departments of Obstetrics and Gynecology, School of Medicine, University of Santiago de Compostela
Departments of Morphological Sciences, School of Medicine, University of Santiago de Compostela


  1. Sendon-Lago J, Seoane S, Eiro N, Bermudez MA, Macia M, Garcia-Caballero T, Vizoso FJ, Perez-Fernandez R. Cancer progression by breast tumors with Pit-1-overexpression is blocked by inhibition of Metalloproteinase (MMP)-13. Breast Cancer Res. 2014;16:505.View ArticlePubMedPubMed CentralGoogle Scholar


© The Author(s). 2017