Specificity of anti-human lysophosphatidic acid receptor 2 (LPA2) antibody. The cross-reactivity of anti-human LPA2 (clone 10D5) was examined by Western blotting. Cell lysate of HeLa cells transfected with human lysophosphatidic acid receptor 1 (LPA1; lane1), LPA2 (lane 2), LPA3 (lane 3), or empty vector (lane4) were loaded onto SDS-PAGE. After the proteins were transferred onto nitrocellulose membrane, the LPA2 protein was detected using antihuman LPA2 antibody (clone 10D5) in an enhanced chemiluminescence (ECL) system. 10D5 did not exhibit cross-reactivity to LPA1 or LPA3.