- Erratum
- Published:
Erratum to: A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation
Breast Cancer Research volume 14, Article number: 302 (2012)
Correction
Following publication of our article [1], the authors noticed that the data incorporated into Figure 4f (1) was incorrect. The first column should be changed from 44, 13 and 46 to read 43, 13 and 44 instead. A corrected version of Figure 4f (1) can be found overleaf.
Mdm2 knockdown in estrogen-treated MCF-7 cells inhibits cell proliferation. Clonal MCF-7 cell lines with mdm2 shRNA or control vector were treated with 2 μg/ml doxycycline (DOX) for three days to induce shRNA expression, followed by 10 nM estrogen (E2) for five days in the presence of DOX. (a) Western blot analysis of Mdm2, p53, p21 and Actin protein levels from whole cell lysates. (b) Number of cells was determined by Guava Viacount assay. 10,000 cells were seeded at beginning of treatments. (c) Fluorescence activated cell sorting (FACS). Cells were harvested, fixed in 30% ethanol, and cellular DNA was stained with propidium iodide. (d) Western blot analysis of Bcl-2 and Actin protein levels from whole cell lysates. (e) MCF-7 cells, grown in matrigel for three weeks, formed mass structures of three sizes: large, intermediate and small. (f) MCF-7 cells, grown in matrigel for three weeks in the presence of 10 nM estrogen and in the absence or presence of 2 μg/ml doxycycline, were fixed and stained with propidium iodide. Masses of different sizes (large, intermediate and small) were counted and presented as percent of the total population. On average, a total of 300 masses were counted. Averages of three independent experiments are shown. The significance in the percent change of large and small masses was determined by the Student t-test (P < 0.05).
References
Brekman A, Singh KE, Polotskaia A, Kundu N, Bargonetti J: A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation. Breast Cancer Research. 2011, 13: R3-10.1186/bcr2804.
Author information
Authors and Affiliations
Corresponding author
Additional information
Competing interests
The authors declare that they have no competing interests.
The online version of the original article can be found at 10.1186/bcr2804
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
Rights and permissions
About this article
Cite this article
Brekman, A., Singh, K.E., Polotskaia, A. et al. Erratum to: A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation. Breast Cancer Res 14, 302 (2012). https://doi.org/10.1186/bcr3130
Published:
DOI: https://doi.org/10.1186/bcr3130