Nestin and collagen triple helix repeat containing 1 in breast cancer progression
© Kharaishvili et al. 2011
Published: 16 November 2011
We have shown that nestin expression is higher in breast carcinoma with a basal phenotype  and collagen triple helix repeat containing 1 (CTHRC1) and periostin may predict bone metastasis of breast cancer . Our aim therefore was to examine the simultaneous role of nestin and CTHRC1 in breast cancer progression.
Archival formalin-fixed paraffin-embedded 173 invasive breast cancer samples classified into WHO histotypes and luminal, triple-negative and Her2 subtypes were immunohistochemically stained with CTHRC1, periostin, nestin and vimentin antibodies. Staining was evaluated with histoscore and neoangiogenesis was assessed as the number of nestin-positive new vessels. The degree of inflammation was evaluated on HE-stained slides. Data were statistically processed by nonparametric Mann-Whitney U test, Spearman correlation coefficient and Pearson chi-square.
Both CTHRC1 stromal (P = 0.013) and nestin epithelial expression (P = 0.001) were higher in the triple-negative subtype. We found strong association between nestin expression in cancer cells and CTHRC1 stromal expression in advanced stage patients (r = 0.614; P = 0.007). Nestin expression was also associated with vimentin expression in breast cancer cells (r = 0.491; P < 0.001). Both nestin and vimentin showed positive association with degree of inflammation, in particular in triple-negative patients (r φ = 0.422; r = 0.521; both P < 0.001). We observed higher nestin positivity in patients with lymph node metastases and high periostin stromal expression (P = 0.031).
For the first time, we report an association between CTHRC1 and nestin expression in patients with advanced breast cancer. Further investigation is needed to better clarify their role in inflammation and breast cancer progression.
This work was supported by grants NS 9956-4 and MSM 6198959216.
- Kolar Z, Ehrmann J, Turashvili G, Bouchal J, Mokry J: A novel myoepithelial/progenitor cell marker in the breast?. Virchows Arch. 2007, 450: 607-609. 10.1007/s00428-007-0403-x.View ArticlePubMedGoogle Scholar
- Kharaishvili G, Cizkova M, Bouchalova K, Mgebrishvili G, Kolar Z, Bouchal J: Collagen triple helix repeat containing 1 protein, periostin and versican in primary and metastatic breast cancer: an immunohistochemical study. J Clin Pathol. 2011Google Scholar