The anoikis suppressing neurotrophic receptor tyrosine kinase 2 ( NTRK2 or TrkB ) is directly targeted and down-regulated by miR-200c. (a) The region of the 3' UTR where miR-200c is predicted to bind. Hec50 cells treated and luciferase assay performed. Columns, mean of five replicates, bars, standard deviation of the mean. ANOVA with Tukey-Kramer post-hoc test, ** P < 0.01. (b) (Right) Immunoblot for TrkB and α-tubulin (loading control) expression. (Left) Quantitation of TrkB integrated intensity (I.I.), normalized to α-tubulin and presented relative to mock. ANOVA, Φ P < 0.05.