Down-regulation of FN1 contributes to miR-200c mediated suppression of migration. Cells were transfected with empty vector (EV) or FN1 and 24 hrs later with miRNA constructs. BT549 (left) and AN3CA (right) cells were treated with mitomycin C and given 24 or 48 hrs, respectively, to migrate. (a) Brightfield images of crystal violet stained cells, dashed black lines indicate edges of the wound immediately after wounding. Scale bars are 100 μm. (b) Quantitation of migratory ability of cells. Columns, mean of five replicates, bars, standard deviation of the mean. ANOVA, ** P < 0.01, Tukey-Kramer post-hoc test, ΦΦ P < 0.01. (c) Immunoblot for FN1, E-cadherin and α-tubulin (loading control).