The extracellular matrix protein fibronectin ( FN1 ) is directly targeted and down-regulated by miR-200c. (a) Regions of the 3' UTR where miR-200c is predicted to bind. Hec50 cells treated and luciferase assay performed. Columns, mean of five replicates, bars, standard deviation of the mean. ANOVA with Tukey-Kramer post-hoc test, ** P < 0.01. (b) Immunoblot for FN1 and α-tubulin (loading control) expression.