Restoration of miR-200c decreases EMT associated genes. (a) Cells were treated with transfection reagent only (mock), scrambled negative control (neg) or miR-200c mimic (200c). RNA was harvested after 72 hrs and qRT-PCR was performed for miR-200c. Samples are normalized to 18S rRNA and presented relative to mock. Columns, mean of three biological replicates, bars, standard deviation of the mean. (b) Heatmap of genes statistically significantly affected by restoration of miR-200c to Hec50 cells and bioinformatically predicted to be targeted by miR-200c.