Key signalling nodes in mammary gland development and cancer: Myc
Breast Cancer Research volume 11, Article number: 210 (2009)
Myc has been intensely studied since its discovery more than 25 years ago. Insight has been gained into Myc's function in normal physiology, where its role appears to be organ specific, and in cancer where many mechanisms contribute to aberrant Myc expression. Numerous signals and pathways converge on Myc, which in turn acts on a continuously growing number of identified targets, via transcriptional and nontranscriptional mechanisms. This review will concentrate on Myc as a signaling mediator in the mammary gland, discussing its regulation and function during normal development, as well as its activation and roles in breast cancer.
Since the early 1980s, numerous investigations have focused on c-Myc to explore its role in normal organ physiology, as well as in tumor biology [1, 2]. The focus of the present review, c-Myc (hereafter referred to as Myc), is the cellular homolog of the avian retroviral oncogene v-myc and, together with N-myc and L-myc, comprises the family of myc proto-oncogenes. The half-lives of Myc mRNA and protein are short, allowing for tight and rapid regulation of Myc levels, which occurs via numerous transcription factors (TFs) and signaling pathways. Proteins that directly bind the promoter or indirectly influence promoter activity have been reviewed recently . To provide some insight into the complexity of Myc regulation, we will mention a few of the factors and pathways that impact on its expression, many of which were shown to be essential during mammary gland development .
The myc promoter contains TF binding sites for Myc (auto-suppression), estrogen receptor (ER) alpha, T-cell factor (TCF) 4, Notch/C promoter-binding factor 1 (Cbf1), E2F, Fos/Jun, signal transducer and activator of transcription (Stat) 3, NF-κB, Smads and others. TFs that occupy or regulate the myc promoter without specific binding sites include p53, CCAAT/enhancer binding protein beta, and Stat5. Moreover, numerous signaling pathways that are frequently deregulated in human cancer influence myc expression; for example, rat sarcoma (Ras)/extracellular signal-related kinase (Erk) and phosphoinositide 3-kinase (PI3K)/serine/threonine kinase Akt (Akt). Post-translational modifications of Myc include phos-phorylation, ubiquitinylation and acetylation, and their effects on Myc activity have been reviewed .
The Myc protein is a basic helix-loop-helix TF that must heterodimerize with the abundantly expressed Max to regulate transcription. Myc-Max dimers bind to hexameric DNA sequences (E-box) and activate transcription by recruiting multiple coactivators . In contrast, when dimerized with basic helix-loop-helix proteins such as Mad or Mnt, Max binds to E-boxes but represses transcription. Myc can also act as a transcriptional repressor via different mechanisms, often involving interaction with Miz1 (for reviews of Myc transcriptional activity, see [1, 6]). It is now well accepted that Myc acts as a relatively weak activator of RNA polymerase II-driven transcription for a large set of target genes, thereby affecting cell cycle, cell growth and metabolism, cell death, adhesion, angiogenesis and other functions (to date, almost 1,700 targets on Myc Cancer Gene [7, 8]). Furthermore, Myc affects RNA polymerase I- and III-mediated transcription , thus regulating ribosome biogenesis and translation. Nontranscriptional roles for Myc in DNA replication and in translation have also been reported recently .
Considering the large number of receptors, hormones, paracrine factors and other signaling molecules that can impact on Myc levels, it is likely that Myc has a variety of functions throughout normal mammary gland development, downstream of one or more of these inputs. To date, however, there are only a few studies on the physiological role of Myc in the mammary gland. In this review we present what is known about Myc from transgenics and conditional knockout models, and also include indirect evidence implicating Myc based on results from other studies. We shall discuss the potential inputs activating Myc during development and the ensuing outputs of Myc activity. A summary of the discussion on the role of Myc in normal development is shown in Figure 1.
Development of the mammary gland begins at embryonic day 10 as an appendix of the ventral skin, with formation of the milk line followed by appearance of placodes . The ErbB4 ligand, Neuregulin3 (Nrg3), was identified as a specification signal for placode formation. Based on this, Keratin14 (K14)-Nrg3 transgenic mice expressing Nrg3 throughout the basal layer of the epidermis, including the stem and progenitor cells, were investigated . Ectopic Nrg3 expression resulted in hyperplastic epidermis and formation of supernumerary placodes. Interestingly, the skin of K14-Nrg3 mice displayed increased Myc expression and decreased levels of α6-integrin and β1-integrin, which are adhesion receptors highly expressed in adult mammary stem cells (SCs) . While the direct stimulus of Myc expression is unknown, it seems to be an important mediator of the phenotype observed in K14-Nrg3 mice, as strong similarities were found in a K14-Myc model where Myc is activated in the epidermis . Nrg3 therefore possibly has a role in promoting mammary lineage commitment and in the regulation of SC fate via Myc.
Mammary stem cells
While these results suggest a role for Myc in embryonic development, its role in adult mammary SCs has not yet been analyzed. A function for Myc in mammary SCs seems likely, however, based on its role in other well-characterized models [1, 13]. In the hematopoietic system, the balance between SC self-renewal and differentiation is controlled by Myc levels, which in turn regulate expression of adhesion molecules such as N-cadherin and β1-integrin . In addition, the Wnt and Notch signaling pathways have been proposed to play important roles in mammary SCs , and their effector proteins - β-catenin/TCF and Notch intracellular domain (NIC)/Cbf1, respectively - each have binding sites on the Myc promoter . Furthermore, a transgenic model expressing stabilized β-catenin in basal cells, which are believed to contain the SC population , displayed upregulation of Myc . Finally, the HC11 mammary epithelial cell line, which has SC-like properties, might be an interesting model to explore Myc function, since Myc levels are downregulated when these cells are induced to differentiate .
Puberty and pregnancy
The steroid hormones estrogen and progesterone, as well as the prolactin receptor binding peptides prolactin and placental lactogen, dominate the extensive developmental changes occurring during puberty and pregnancy [4, 17]. Both estrogen and progesterone are able to directly stimulate Myc expression via an estrogen response element  and a progesterone receptor regulatory element . While Myc is expressed at low levels in prepubertal and virgin glands, it reaches its highest expression levels between days 6.5 and 12.5 of pregnancy, after which the RNA level slowly returns to baseline until parturition .
Interestingly, Myc was shown to be directly downstream of estrogen and progesterone in breast cancer cells, stimulating their proliferation [21, 22], but one of the major differences between normal breast and malignant breast is that the estrogen- and progesterone receptor-positive cells do not proliferate during normal development. Instead, by producing paracrine mediators, estrogen and progesterone stimulate proliferation of neighboring cells via amphiregulin  and Wnt4 , respectively. Other growth factors, such as epidermal growth factor or receptor activator of NF-κB ligand, have also been shown to act as paracrine mediators during mammary gland development ; and importantly, Wnt, epidermal growth factor and receptor activator of NF-κB ligand might all affect Myc levels directly or indirectly .
Although no study has yet addressed the contribution of Myc to pubertal development and early pregnancy, it is very likely that Myc is induced via paracrine signals, and, at least early in pregnancy when Myc levels are highest, Myc might promote proliferation. Importantly, different Myc targets such as cyclin-dependent kinase (Cdk) 4, nucleophosmin and nucleolin are also highly expressed at this time  - prompting us to speculate that Myc might not only have a direct role in proliferation, but also in the synthesis of ribosomal components required for rapid growth during pregnancy.
A transgenic model revealed why it is important for Myc levels to decrease, starting at 12.5 days of pregnancy, and to remain low until parturition. Using a doxycycline inducible model (MMTV-rtTA/TetO-MYC (MTB/TOM)) , it was shown that transient overexpression of Myc between days 12.5 and 15.5 of pregnancy induced a lactation failure . Abnormal Myc expression was shown not only to induce proliferation, but also to promote precocious Stat5 activation and differentiation, followed by premature involution, triggered by milk stasis. Decreased levels of Caveolin1 (Cav1), a direct target of Myc repression , were shown to be responsible for the phenotype. Cav1 is a negative regulator of Janus kinase2-Stat5 signaling, and Cav1-/-mammary epithelial cells show hyperactivation of Stat5 and spontaneous milk production , similar to what is observed when Myc is elevated late in pregnancy.
These results demonstrate an important characteristic of Myc - namely, the effects of Myc are dependent upon the developmental stage of the mammary gland. Myc overexpression between 12.5 and 15.5 days of pregnancy was necessary and sufficient to induce the observed phenotype, while overexpression during other short intervals (for example, 9.5 to 12.5 days) did not result in lactation failure . Deregulated Myc therefore leads to a premature decrease in Cav1, thereby removing its restraining influence on prolactin receptor-Janus kinase2-Stat5 signaling.
In many cell types Myc is downregulated when cells undergo terminal differentiation. Indeed, Myc RNA levels do drop during lactation to below those levels found in the virgin gland ; however, the molecular reason for this dramatic decrease is not known. The mammary gland as a milk factory produces immense amounts of lipids, lactose and proteins, and most of its energy is devoted to milk component synthesis. Considering the importance of Myc in energy and glucose metabolism as well as ribosome biogenesis and translation , it is possible that, even despite its low levels, Myc has an essential function during lactation.
Indeed, data from our laboratory revealed a novel role for Myc in the mammary gland using a conditional knockout approach. In c-mycfl/flWAP(whey acidic protein)iCre mice, loss of Myc occurs exclusively in luminal alveolar cells starting from mid-pregnancy. We show that milk production was reduced in Myc mutant mothers, while milk composition was unchanged between wild-type and mutant mothers . Electron microscopy revealed that there were less secretory vesicles budding from the endoplasmic reticulum in lactating mutant cells, suggesting a decreased protein synthesis. In polysomal fractionation experiments we found that translation efficiency was generally decreased in lactating, Myc-deficient mammary glands. Furthermore, we observed reduced expression levels of ribosomal proteins and RNA, as well as proteins involved in translation and ribosome biogenesis. Although compensation by N-Myc or L-Myc cannot be excluded, neither was found upregulated in c-Myc mutant glands. These results highlight Myc's importance for mammary gland function even when endogenous levels are low.
The impact of Myc on apoptosis has been widely studied in many systems . In the mammary gland, the high levels of apoptosis during the first phase of involution are promoted by the leukemia inhibitory factor-Stat3 axis . Compared with its low expression in lactating glands, higher Myc levels are detected during involution . Importantly, a role for Myc during the first apoptotic phase was uncovered in mice with a conditional deletion of Socs3 (Socs3-/flWAPiCre), a negative regulator of leukemia inhibitory factor-Stat3 signaling . Socs3-deficient glands displayed accelerated apoptosis accompanied by elevated levels of p-Stat3 and Myc, which is a direct Stat3 target gene.
To further analyze Myc's function in apoptosis, the doxycycline-inducible MTB/TOM model described above  was used, giving more evidence for a direct role of Myc in involution. Overexpression of Myc prior to forced weaning caused a dramatic acceleration of involution, accompanied by increased apoptosis and high levels of the pro-apoptotic proteins Bax, E2F-1 and p53, which have all been described as direct or indirect Myc targets.
The conclusions based on those two models suggest that Myc acts as a central mediator of apoptotic signaling in the mammary gland, being a direct target of Stat3 and inducing expression of pro-apoptotic genes.
Background on Myc in breast cancer
Alterations in Myc have been found in many types of tumors. At the genomic level these include gene amplification, chromosomal translocations and point mutations. Furthermore, Myc is regulated by multiple signals that control promoter activity, transcriptional elongation and translation, as well as by post-translational modifications that control Myc's transcriptional targets as well as protein stability. Since most tumors have numerous alterations in signaling cascades, Myc is likely to be deregulated by some mechanisms in most cancers.
Considering breast cancer, amplification is the most commonly described alteration. The MYC amplicon on chromosome 8q23-24 was one of the first consistent genetic alterations found . Results from a meta-analysis of breast tumors yielded a frequency of 15.7% for the MYC amplicon, with a range of 4 to 52% depending on the study [21, 33]. MYC amplification is found in a high proportion of tumors with Brca1 alterations, as well as in ERα-negative, basal-like tumors [34, 35]. Despite intense screening efforts, point mutations of Myc have not been described in breast or other carcinomas . Other mechanisms promoting increased Myc levels, however, have been found. The ubiquitin ligase F-box and WB repeat domain containing 7, which catalyzes polyubiquitination of Myc and ensuing degradation, is often mutated or downregulated in breast cancer . Moreover, the de-ubiquitinating enzyme ubiquitin-specific protease 28-which antagonizes F-box and WB repeat domain containing 7, thereby stabilizing Myc - has been found overexpressed in a small panel of breast tumors . In addition to these breast tumor-specific alterations, it is possible that Myc is deregulated in most breast tumors since the normal tight control that is exerted on Myc at multiple levels is impaired in essentially all cancer cells.
What is the output of deregulated Myc in breast cancer? Myc levels respond to proliferative and anti-proliferative stimuli, and many reported Myc target genes, such as cyclin D2, Cdk4 and the Cdk inhibitor p21Cip1, are important regulators of proliferation . A major mechanism underlying Myc's role in breast cancer is activation of cyclin E-Cdk2 via repression of p21Cip1 . Myc deregulation not only impacts on proliferation, but on various other processes like survival and apoptosis.
Here we would like to mention Myc and translational control, since we have recently shown an important role for Myc in translation also during mammary gland development . Generation of the Eμ-Myc B-cell leukemia model in mice with heterozygosity in the gene encoding the L24 ribosomal protein restored normal levels of protein synthesis in the leukemic cells, thereby suppressing Myc's oncogenic potential . These results show that, in addition to proliferative effects, abnormal Myc activation also deregulates protein synthesis, which in this model is necessary for oncogenesis.
Considering the broad-ranging effects of Myc, the outcome of its activation in breast cancer is likely to be dependent upon the cellular context. Indeed, using a siRNA approach to knockdown Myc in a panel of breast cancer cell lines, and combining this with a genomic and phenotypic analysis, revealed that selectively regulated target genes in each cell line were responsible for the differential effects resulting from Myc loss. A comprehensive list of potential Myc targets in the BT-474, MCF-7 and MDA-MB-231 breast cancer cell lines can be found in Cappellen and colleagues' study . A summary of the discussion on the role of Myc in cancer is shown in Figure 2.
Myc and mammary cancer
Myc was the first oncogene tested for mammary tumor-forming potential using the mouse mammary tumor virus (MMTV)-long terminal repeat to drive its expression. Tumor incidence in MMTV-Myc transgenic females was high; however, the kinetics of tumor appearance suggested that Myc expression alone was not sufficient to induce cancer . Indeed, double transgenics expressing Myc and mutant Hras showed more rapid mammary tumor development . More recently, it was shown that Myc induction using the inducible MTB/TOM model described above  results in mammary tumors, with approximately one-half also harboring activating Kras2 mutations. Interestingly, these tumors did not regress following Myc deinduction, demonstrating that Hras mutations not only change tumor kinetics but also cause progression to Myc independency .
Myc and Notch
Each membrane spanning Notch receptor is proteolytically processed in response to ligand binding, releasing NIC, which converts the nuclear Cbf1 repressor to a transcriptional activator. A link between aberrant Notch signaling and mammary cancer was first discovered in MMTV-induced tumors with proviral DNA integrated within the Notch4 gene, leading to constitutive NIC expression. Different mechanisms activate Notch signaling in human breast cancer [42, 43]. For example, coexpression of Jagged1 ligand and Notch receptors has been found in breast cancers, in particular the triple-negative (negative for ERα, for progesterone receptor and for ErbB2) subtype , suggesting an autocrine mechanism of Notch pathway activation. Furthermore, levels of Numb, a negative regulator of Notch, were shown to be low in ~50% of primary breast tumors , which could contribute to maintenance of pathway activity.
Based on the observation that mammary tumors in the MMTV-NICtransgenics presented elevated Myc, its role in Notch transformation was examined in mice with floxed Myc alleles . Conditional ablation of Myc using the WAPCre transgene revealed that Myc was indispensable for development of NIC-driven mammary tumors. This contribution of Myc to Notch-induced tumorigenesis is interesting especially when comparing it with Wnt pathway-driven models (see below). Moreover, Myc was shown to be a direct target of the Notch pathway, since a complex of NIC and Cbf1 was detected on the Myc promoter. The Cbf1 binding site on the human Myc promoter is conserved, and immunohisto-chemistry revealed that there was a significant correlation between high Myc levels and NIC in human breast tumors . It is intriguing that coexpression of Jagged ligand and Notch receptors is found in triple-negative breast tumors , a subgroup that also has high Myc activity .
Myc and the Wnt pathway
Wnt1 was the first identified oncogene activated by MMTV insertional mutagenesis. Wnt-mediated activation of the canonical pathway leads to β-catenin stabilization, TCF binding and transcriptional activation of Myc. Mammary tumors arising in Wnt1 transgenics  and models driven by a β-catenin-stabilized mutant  show elevated levels of Myc. Human breast tumors, unlike colon cancer, do not possess Wnt pathway-activating mutations. Deregulation of Wnt signaling appears to occur by autocrine mechanisms, however, since multiple Wnt ligands and Frizzled receptors are coexpressed , and the negative Wnt pathway regulator - secreted Frizzled-related protein (sFRP1) - is often absent . A positive feedback loop has also been described for Myc and the Wnt pathway. In telomerase-immortalized, Myc-transformed human mammary epithelial cells, Myc was shown to repress sFRP1 and Dickkopf 1, another negative pathway regulator, thereby contributing to activation of canonical Wnt signaling . Along the same lines, Myc knockdown in MDA-MB-231 tumor cells increased Dickkopf 3 expression .
What is Myc's role in tumors induced by Wnt pathway activation? The dependency of Wnt-driven mouse mammary tumors on Myc expression has not been tested. In other tumor models driven by adenomatosis polyposis coli (APC) loss, the importance of Myc has been examined using organ-specific Cre recombinase-mediated deletion of floxed Myc alleles. In the intestines, Myc deletion reversed the tumor phenotype induced by APC loss, and it was shown that the majority of Wnt targets in the intestines were Myc dependent . In striking contrast, Myc deletion had no affect on the phenotype of APC loss in the liver, where most Wnt target genes were β-catenin dependent but Myc independent .
These two studies [51, 52] reveal that the importance of Myc in a particular tumor model is very specific and can differ as already discussed for the normal organ-specific Myc functions. As discussed above, deregulated Myc might enforce autocrine Wnt pathway activity in human tumors by repressing negative regulators such as sFRP1. Other potential roles for Myc have not been examined; however, blockade of the Wnt pathway usually results in lowering Myc levels. Stable expression of sFRP1 in MDA-MB-157 and MDA-MB-231 breast tumor cell lines blocks proliferation of both cell lines, and Myc RNA was decreased in the former  while Myc protein was lower in the latter . In a panel of breast cancer cell lines, siRNA-mediated knockdown of Dishevelled, an essential mediator of Wnt signaling, led to lower Myc and decreased proliferation in most cell lines .
In summary, the current data suggest that, in mouse and human mammary tumors with constitutive Wnt signaling, Myc levels are elevated and might have a role in transformation.
Myc and ErbB2
Amplification of ERBB2 leading to receptor overexpression is found in 20 to 25% of primary breast tumors. In these tumors, constitutive ErbB2 activation stimulates numerous intra-cellular signaling pathways including Ras/Erk and PI3K/Akt, both of which impact on Myc transcription and protein stability. The role of Myc has been examined in the ErbB2-overexpressing SKBr3 and BT-474 breast tumor cell lines. Treatment of both with the ErbB2-specific antibody trastuzu-mab caused a cell cycle block that was accompanied by a decrease in PI3K/Akt pathway activity, and by downregulation of Myc and D-type cyclins . Interestingly, ectopic expression of Myc in SKBr3 cells partially rescued the cells from functional ErbB2 inactivation , pointing to the importance of Myc as an ErbB2 effector.
Myc and estrogen receptor alpha
In the normal breast, both rodent and human, a major role of the ERα-positive cells is to act as sensors to relay a proliferative signal to neighboring cells. In contrast, many breast tumor cells are ERα-positive, and they have not only acquired the potential to proliferate in response to steroid hormones but are also dependent upon them for survival [21, 57]. Since Myc is an ERα target, it is important to understand whether Myc has a role in acquisition of this phenotype. Moreover, since patients whose tumors are ERα-positive are treated with anti-estrogen therapy, Myc's role in response or resistance is also of high interest. Unfortunately, consistent clinical data relating MYC amplification or expression levels to endocrine therapy response are not available . Overexpression of ErbB2 has been correlated with de novo and acquired endocrine resistance , however, and Myc is an ErbB2 effector.
The role of Myc in ERα signaling has been well characterized in the MCF-7 breast tumor cell line . Myc RNA increases rapidly in response to estrogen treatment of these cells, and Myc knockdown impairs the ability of estrogens to stimulate proliferation. Furthermore, Myc overexpression in cells arrested by ERα antagonists overcomes the proliferative block. Interestingly, adaptation of MCF-7 cells to growth in estrogen-deprived medium is associated with upregulation of ERα-regulated target genes, including Myc , suggesting a mechanism whereby Myc maintains an important role in proliferation and survival even in the absence of ERα activity. It should also be mentioned that ERα cross-regulates ErbB2, which in turn impacts on Myc via activation of downstream signaling pathways .
From transcriptome and network analyses, the close link between Myc and ERα signaling has become even more apparent. MCF-7 cells show a high level of overlap between ERα-regulated and Myc-regulated genes. Indeed, more than 50% of the estrogen-responsive genes are also Myc targets . Moreover, a meta-analysis of transcriptional and pathway data, carried out on primary breast tumors, revealed that Myc activity is elevated in ERα-negative, basal-like breast cancers, as measured by target gene levels .
Myc as a prognostic, predictive or therapeutic target in breast cancer
Considering that Myc deregulation is so common in breast cancer, Myc has been examined as a prognostic factor and as a predictive factor. Indeed, MYC amplification is associated with aggressive clinical features including high grade and lymph node positivity, and correlates with poor patient outcome . A major interest in breast cancer is the use of genetic alterations to categorize patients into treatment groups; a good example being detection of the ERBB2 amplicon for trastuzumab treatment. Gene expression signatures are also being generated in order to provide predictive data on patient response to standard chemotherapeutics and to targeted therapy. In one study of chemotherapy-treated breast cancer patients, an attempt to correlate Myc pathway activity with response yielded inconsistent results. The group with Myc and Ras pathway activation had a high percentage of responders, while patients whose tumors had Myc and E2F pathway activity responded poorly . These results underscore the difficulty of using Myc levels alone as a predictive or prognostic factor, and emphasize the fact that the cellular context of Myc expression and activity determine the outcome.
Approaches to target Myc activity in tumors are also under consideration [34, 61]. Although targeting Myc is appealing, there are many difficulties associated with altering transcription factor activity. Considering the variety of kinase inhibitors currently available, it is worth considering their use in breast tumors with Myc deregulation. Based on the molecular concepts analysis  of the Myc pathway activation signature in cancer, and on the identification of the genes downregulated in MCF-7 cells treated with wortmannin and LY-294002, there is reason to believe that PI3K inhibitors might be especially potent in breast cancers with high Myc activity. As discussed , ERα-negative, basal-like breast tumors with high Myc activity might be particularly susceptible to PI3K inhibitors.
Clearly, there are numerous signaling events in the development of the mammary gland that can be mediated, at least in part, via Myc. Using transgenic models, functions for Myc - some potential, others data based - have been discussed for embryonic development, pregnancy, lactation and involution. Furthermore, results from other model systems suggest that Myc could play a role in stem cell fate, as well as during early pregnancy, where Myc expression levels are highest in the normal gland. Even more efforts have been made to investigate Myc's role in transformation, as deregulation of Myc via amplification, overexpression or stabilization of the protein is a frequent event in breast cancer. Signaling pathways implicated in breast cancer, such as ERα, ErbB2, Notch and Wnt, all contribute to aberrant Myc levels or activity. The challenge for future studies will be to reveal the suitability of targeting Myc for breast cancer treatment, either by direct inhibition or by indirectly targeting another pathway.
This article is part of a review series on Key signalling nodes in mammary gland development and cancer, edited by Adrian Lee and Charles Streuli.
Other articles in the series can be found online at: http://breast-cancer-research.com/series/bcr_signalling_nodes
serine/threonine kinase Akt
adenomatosis polyposis coli
C promoter-binding factor 1
extracellular signal-related kinase
mouse mammary tumor virus
Notch intracellular domain
secreted Frizzled-related protein 1
signal transducer and activator of transcription
Eilers M, Eisenman RN: Myc's broad reach. Genes Dev. 2008, 22: 2755-2766. 10.1101/gad.1712408.
Meyer N, Penn LZ: Reflecting on 25 years with MYC. Nat Rev Cancer. 2008, 8: 976-990. 10.1038/nrc2231.
Wierstra I, Alves J: The c-myc promoter: still MysterY and challenge. Adv Cancer Res. 2008, 99: 113-333. full_text.
Hennighausen L, Robinson GW: Information networks in the mammary gland. Nat Rev Mol Cell Biol. 2005, 6: 715-725. 10.1038/nrm1714.
Vervoorts J, Luscher-Firzlaff J, Luscher B: The ins and outs of MYC regulation by posttranslational mechanisms. J Biol Chem. 2006, 281: 34725-34729. 10.1074/jbc.R600017200.
Grandori C, Cowley SM, James LP, Eisenman RN: The Myc/Max/Mad network and the transcriptional control of cell behavior. Annu Rev Cell Dev Biol. 2000, 16: 653-699. 10.1146/annurev.cellbio.16.1.653.
Myc Cancer Gene. [http://www.myc-cancer-gene.org/index.asp]
Zeller KI, Jegga AG, Aronow BJ, O'Donnell KA, Dang CV: An integrated database of genes responsive to the Myc oncogenic transcription factor: identification of direct genomic targets. Genome Biol. 2003, 4: R69-10.1186/gb-2003-4-10-r69.
Cole MD, Cowling VH: Transcription-independent functions of MYC: regulation of translation and DNA replication. Nat Rev Mol Cell Biol. 2008, 9: 810-815. 10.1038/nrm2467.
Robinson GW: Cooperation of signalling pathways in embryonic mammary gland development. Nat Rev Genet. 2007, 8: 963-972. 10.1038/nrg2227.
Howard BA: The role of NRG3 in mammary development. J Mammary Gland Biol Neoplasia. 2008, 13: 195-203. 10.1007/s10911-008-9082-8.
Visvader JE, Lindeman GJ: Mammary stem cells and mam-mopoiesis. Cancer Res. 2006, 66: 9798-9801. 10.1158/0008-5472.CAN-06-2254.
Tanos T, Brisken C: What signals operate in the mammary niche?. Breast Dis. 2008, 29: 69-82.
Wilson A, Murphy MJ, Oskarsson T, Kaloulis K, Bettess MD, Oser GM, Pasche AC, Knabenhans C, Macdonald HR, Trumpp A: c-Myc controls the balance between hematopoietic stem cell self-renewal and differentiation. Genes Dev. 2004, 18: 2747-2763. 10.1101/gad.313104.
Teuliere J, Faraldo MM, Deugnier MA, Shtutman M, Ben-Ze'ev A, Thiery JP, Glukhova MA: Targeted activation of beta-catenin signaling in basal mammary epithelial cells affects mammary development and leads to hyperplasia. Development. 2005, 132: 267-277. 10.1242/dev.01583.
Williams C, Helguero L, Edvardsson K, Haldosen LA, Gustafsson JA: Gene expression in murine mammary epithelial stem cell-like cells shows similarities to human breast cancer gene expression. Breast Cancer Res. 2009, 11: R26-10.1186/bcr2256.
Hovey RC, Trott JF, Vonderhaar BK: Establishing a framework for the functional mammary gland: from endocrinology to morphology. J Mammary Gland Biol Neoplasia. 2002, 7: 17-38. 10.1023/A:1015766322258.
Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF, Wang Q, Bekiranov S, Sementchenko V, Fox EA, Silver PA, Gingeras TR, Liu XS, Brown M: Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006, 38: 1289-1297. 10.1038/ng1901.
Moore MR, Zhou JL, Blankenship KA, Strobl JS, Edwards DP, Gentry RN: A sequence in the 5' flanking region confers prog-estin responsiveness on the human c-myc gene. J Steroid Biochem Mol Biol. 1997, 62: 243-252. 10.1016/S0960-0760(97)00036-8.
Master SR, Hartman JL, D'Cruz CM, Moody SE, Keiper EA, Ha SI, Cox JD, Belka GK, Chodosh LA: Functional microarray analysis of mammary organogenesis reveals a developmental role in adaptive thermogenesis. Mol Endocrinol. 2002, 16: 1185-1203. 10.1210/me.16.6.1185.
Butt AJ, Caldon CE, McNeil CM, Swarbrick A, Musgrove EA, Sutherland RL: Cell cycle machinery: links with genesis and treatment of breast cancer. Adv Exp Med Biol. 2008, 630: 189-205.
Sutherland RL, Prall OW, Watts CK, Musgrove EA: Estrogen and progestin regulation of cell cycle progression. J Mammary Gland Biol Neoplasia. 1998, 3: 63-72. 10.1023/A:1018774302092.
Ciarloni L, Mallepell S, Brisken C: Amphiregulin is an essential mediator of estrogen receptor alpha function in mammary gland development. Proc Natl Acad Sci USA. 2007, 104: 5455-5460. 10.1073/pnas.0611647104.
Brisken C, Heineman A, Chavarria T, Elenbaas B, Tan J, Dey SK, McMahon JA, McMahon AP, Weinberg RA: Essential function of Wnt-4 in mammary gland development downstream of prog-esterone signaling. Genes Dev. 2000, 14: 650-654.
Rosen JM: Hormone receptor patterning plays a critical role in normal lobuloalveolar development and breast cancer progression. Breast Dis. 2003, 18: 3-9.
D'Cruz CM, Gunther EJ, Boxer RB, Hartman JL, Sintasath L, Moody SE, Cox JD, Ha SI, Belka GK, Golant A, Cardiff RD, Chodosh LA: c-MYC induces mammary tumorigenesis by means of a preferred pathway involving spontaneous Kras2 mutations. Nat Med. 2001, 7: 235-239. 10.1038/84691.
Blakely CM, Sintasath L, D'Cruz CM, Hahn KT, Dugan KD, Belka GK, Chodosh LA: Developmental stage determines the effects of MYC in the mammary epithelium. Development. 2005, 132: 1147-1160. 10.1242/dev.01655.
Sotgia F, Schubert W, Pestell RG, Lisanti MP: Genetic ablation of caveolin-1 in mammary epithelial cells increases milk production and hyper-activates STAT5a signaling. Cancer Biol Ther. 2006, 5: 292-297.
Stoelzle T, Schwarb P, Trumpp A, Hynes NE: c-Myc affects mRNA translation, cell proliferation and progenitor cell function in the mammary gland. BMC Biol. 2009, 7: 63-10.1186/1741-7007-7-63.
Watson CJ: Involution: apoptosis and tissue remodelling that convert the mammary gland from milk factory to a quiescent organ. Breast Cancer Res. 2006, 8: 203-10.1186/bcr1401.
Sutherland KD, Vaillant F, Alexander WS, Wintermantel TM, Forrest NC, Holroyd SL, McManus EJ, Schutz G, Watson CJ, Chodosh LA, Lindeman GJ, Visvader JE: c-myc as a mediator of accelerated apoptosis and involution in mammary glands lacking Socs3. EMBO J. 2006, 25: 5805-5815. 10.1038/sj.emboj.7601455.
Escot C, Theillet C, Lidereau R, Spyratos F, Champeme MH, Gest J, Callahan R: Genetic alteration of the c-myc protooncogene (MYC) in human primary breast carcinomas. Proc Natl Acad Sci USA. 1986, 83: 4834-4838. 10.1073/pnas.83.13.4834.
Deming SL, Nass SJ, Dickson RB, Trock BJ: C-myc amplification in breast cancer: a meta-analysis of its occurrence and prognostic relevance. Br J Cancer. 2000, 83: 1688-1695. 10.1054/bjoc.2000.1522.
Chen Y, Olopade OI: MYC in breast tumor progression. Expert Rev Anticancer Ther. 2008, 8: 1689-1698. 10.1586/14737184.108.40.2069.
Nikolsky Y, Sviridov E, Yao J, Dosymbekov D, Ustyansky V, Kaz-nacheev V, Dezso Z, Mulvey L, Macconaill LE, Winckler W, Sere-bryiskaya T, Nikolskaya T, Polyak K: Genome-wide functional synergy between amplified and mutated genes in human breast cancer. Cancer Res. 2008, 68: 9532-9540. 10.1158/0008-5472.CAN-08-3082.
Schulein C, Eilers M: An unsteady scaffold for Myc. EMBO J. 2009, 28: 453-454. 10.1038/emboj.2009.14.
Welcker M, Clurman BE: FBW7 ubiquitin ligase: a tumour suppressor at the crossroads of cell division, growth and differentiation. Nat Rev Cancer. 2008, 8: 83-93. 10.1038/nrc2290.
Popov N, Wanzel M, Madiredjo M, Zhang D, Beijersbergen R, Bernards R, Moll R, Elledge SJ, Eilers M: The ubiquitin-specific protease USP28 is required for MYC stability. Nat Cell Biol. 2007, 9: 765-774. 10.1038/ncb1601.
Barna M, Pusic A, Zollo O, Costa M, Kondrashov N, Rego E, Rao PH, Ruggero D: Suppression of Myc oncogenic activity by ribosomal protein haploinsufficiency. Nature. 2008, 456: 971-975. 10.1038/nature07449.
Cappellen D, Schlange T, Bauer M, Maurer F, Hynes NE: Novel c-MYC target genes mediate differential effects on cell proliferation and migration. EMBO Rep. 2007, 8: 70-76. 10.1038/sj.embor.7400849.
Stewart TA, Pattengale PK, Leder P: Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell. 1984, 38: 627-637. 10.1016/0092-8674(84)90257-5.
Callahan R, Egan SE: Notch signaling in mammary development and oncogenesis. J Mammary Gland Biol Neoplasia. 2004, 9: 145-163. 10.1023/B:JOMG.0000037159.63644.81.
Efstratiadis A, Szabolcs M, Klinakis A: Notch, Myc and breast cancer. Cell Cycle. 2007, 6: 418-429.
Reedijk M, Odorcic S, Chang L, Zhang H, Miller N, McCready DR, Lockwood G, Egan SE: High-level coexpression of JAG1 and NOTCH1 is observed in human breast cancer and is associated with poor overall survival. Cancer Res. 2005, 65: 8530-8537. 10.1158/0008-5472.CAN-05-1069.
Pece S, Serresi M, Santolini E, Capra M, Hulleman E, Galimberti V, Zurrida S, Maisonneuve P, Viale G, Di Fiore PP: Loss of negative regulation by Numb over Notch is relevant to human breast carcinogenesis. J Cell Biol. 2004, 167: 215-221. 10.1083/jcb.200406140.
Alles MC, Gardiner-Garden M, Nott DJ, Wang Y, Foekens JA, Sutherland RL, Musgrove EA, Ormandy CJ: Meta-analysis and gene set enrichment relative to ER status reveal elevated activity of MYC and E2F in the 'basal' breast cancer subgroup. PLoS ONE. 2009, 4: e4710-10.1371/journal.pone.0004710.
Huang S, Li Y, Chen Y, Podsypanina K, Chamorro M, Olshen AB, Desai KV, Tann A, Petersen D, Green JE, Varmus HE: Changes in gene expression during the development of mammary tumors in MMTV-Wnt-1 transgenic mice. Genome Biol. 2005, 6: R84-10.1186/gb-2005-6-10-r84.
Ayyanan A, Civenni G, Ciarloni L, Morel C, Mueller N, Lefort K, Mandinova A, Raffoul W, Fiche M, Dotto GP, Brisken C: Increased Wnt signaling triggers oncogenic conversion of human breast epithelial cells by a Notch-dependent mechanism. Proc Natl Acad Sci USA. 2006, 103: 3799-3804. 10.1073/pnas.0600065103.
Matsuda Y, Schlange T, Oakeley EJ, Boulay A, Hynes NE: WNT signaling enhances breast cancer cell motility and blockade of the WNT pathway by sFRP1 suppresses MDA-MB-231 xenograft growth. Breast Cancer Res. 2009, 11: R32-10.1186/bcr2317.
Cowling VH, D'Cruz CM, Chodosh LA, Cole MD: c-Myc transforms human mammary epithelial cells through repression of the Wnt inhibitors DKK1 and SFRP1. Mol Cell Biol. 2007, 27: 5135-5146. 10.1128/MCB.02282-06.
Sansom OJ, Meniel VS, Muncan V, Phesse TJ, Wilkins JA, Reed KR, Vass JK, Athineos D, Clevers H, Clarke AR: Myc deletion rescues Apc deficiency in the small intestine. Nature. 2007, 446: 676-679. 10.1038/nature05674.
Reed KR, Athineos D, Meniel VS, Wilkins JA, Ridgway RA, Burke ZD, Muncan V, Clarke AR, Sansom OJ: B-catenin deficiency, but not Myc deletion, suppresses the immediate phenotypes of APC loss in the liver. Proc Natl Acad Sci USA. 2008, 105: 18919-18923. 10.1073/pnas.0805778105.
Bafico A, Liu G, Goldin L, Harris V, Aaronson SA: An autocrine mechanism for constitutive Wnt pathway activation in human cancer cells. Cancer Cell. 2004, 6: 497-506. 10.1016/j.ccr.2004.09.032.
Schlange T, Matsuda Y, Lienhard S, Huber A, Hynes NE: Autocrine WNT signaling contributes to breast cancer cell proliferation via the canonical WNT pathway and EGFR transacti-vation. Breast Cancer Res. 2007, 9: R63-10.1186/bcr1769.
Lane HA, Beuvink I, Motoyama AB, Daly JM, Neve RM, Hynes NE: ErbB2 potentiates breast tumor proliferation through modulation of p27(Kip1)-Cdk2 complex formation: receptor overex-pression does not determine growth dependency. Mol Cell Biol. 2000, 20: 3210-3223. 10.1128/MCB.20.9.3210-3223.2000.
Neve RM, Sutterluty H, Pullen N, Lane HA, Daly JM, Krek W, Hynes NE: Effects of oncogenic ErbB2 on G1 cell cycle regulators in breast tumour cells. Oncogene. 2000, 19: 1647-1656. 10.1038/sj.onc.1203470.
Arpino G, Wiechmann L, Osborne CK, Schiff R: Crosstalk between the estrogen receptor and the HER tyrosine kinase receptor family: molecular mechanism and clinical implications for endocrine therapy resistance. Endocr Rev. 2008, 29: 217-233. 10.1210/er.2006-0045.
Jeng MH, Shupnik MA, Bender TP, Westin EH, Bandyopadhyay D, Kumar R, Masamura S, Santen RJ: Estrogen receptor expression and function in long-term estrogen-deprived human breast cancer cells. Endocrinology. 1998, 139: 4164-4174. 10.1210/en.139.10.4164.
Musgrove EA, Sergio CM, Loi S, Inman CK, Anderson LR, Alles MC, Pinese M, Caldon CE, Schutte J, Gardiner-Garden M, Ormandy CJ, McArthur G, Butt AJ, Sutherland RL: Identification of functional networks of estrogen- and c-Myc-responsive genes and their relationship to response to tamoxifen therapy in breast cancer. PLoS ONE. 2008, 3: e2987-10.1371/journal.pone.0002987.
Salter KH, Acharya CR, Walters KS, Redman R, Anguiano A, Garman KS, Anders CK, Mukherjee S, Dressman HK, Barry WT, Marcom KP, Olson J, Nevins JR, Potti A: An integrated approach to the prediction of chemotherapeutic response in patients with breast cancer. PLoS One. 2008, 3: e1908-10.1371/journal.pone.0001908.
Vita M, Henriksson M: The Myc oncoprotein as a therapeutic target for human cancer. Semin Cancer Biol. 2006, 16: 318-330. 10.1016/j.semcancer.2006.07.015.
Rhodes DR, Kalyana-Sundaram S, Tomlins SA, Mahavisno V, Kasper N, Varambally R, Barrette TR, Ghosh D, Varambally S, Chinnaiyan AM: Molecular concepts analysis links tumors, pathways, mechanisms, and drugs. Neoplasia. 2007, 9: 443-454. 10.1593/neo.07292.
The authors are very grateful to G MacDonald (FMI, Basel, Switzerland) for helpful suggestions on the manuscript. The laboratory of NEH is supported by the Friedrich Miescher Institute for Biomedical Research (Basel, Switzerland), part of the Novartis Research Foundation.
The authors declare that they have no competing interests.
About this article
Cite this article
Hynes, N.E., Stoelzle, T. Key signalling nodes in mammary gland development and cancer: Myc. Breast Cancer Res 11, 210 (2009). https://doi.org/10.1186/bcr2406
- Mammary Gland
- Mouse Mammary Tumor Virus
- Mammary Gland Development
- Breast Tumor Cell Line
- HC11 Mammary Epithelial Cell Line