BCoR-L1 variation and breast cancer
Breast Cancer Research volume 9, Article number: R54 (2007)
BRCA1 is involved in numerous essential processes in the cell, and the effects of BRCA1 dysfunction in breast cancer carcinogenesis are well described. Many of the breast cancer susceptibility genes such as BRCA2, p53, ATM, CHEK2, and BRIP1 encode proteins that interact with BRCA1. BCL6 corepressor-like 1 (BCoR-L1) is a newly described BRCA1-interacting protein that displays high homology to several proteins known to be involved in the fundamental processes of DNA damage repair and transcription regulation. BCoR-L1 has been shown to play a role in transcription corepression, and expression of the X-linked BCoR-L1 gene has been reported to be dysregulated in breast cancer subjects. BCoR-L1 is located on the X chromosome and is subject to X inactivation.
We performed mutation analysis of 38 BRCA1/2 mutation-negative breast cancer families with male breast cancer, prostate cancer, and/or haplotype sharing around BCoR-L1 to determine whether there is a role for BCoR-L1 as a high-risk breast cancer predisposition gene. In addition, we conducted quantitative real-time PCR (qRT-PCR) on lymphoblastoid cell lines (LCLs) from the index cases from these families and a number of cancer cell lines to assess the role of BCoR-L1 dysregulation in cancer and cancer families.
Very little variation was detected in the coding region, and qRT-PCR analysis revealed that BCoR-L1 expression is highly variable in cancer-free subjects, high-risk breast cancer patients, and cancer cell lines. We also report the investigation of a new expression control, DIDO1 (death inducer-obliterator 1), that is superior to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and UBC (ubiquitin C) for analysis of expression in LCLs.
Our results suggest that BCoR-L1 expression does not play a large role in predisposition to familial breast cancer.
Less than 40% of familial breast cancer can be attributed to mutations in the high-risk genes BRCA1 and BRCA2 despite their high penetrance [1, 2]. Syndromes displaying a predisposition for breast cancer such as Li-Fraumeni syndrome (resulting from p53 gene mutations) , ataxia telangiectasia (ataxia telangiectasia-mutated, or ATM, gene) , and Cowden syndrome (phosphatase and tensin homologue, or PTEN, gene)  are estimated to account for no more than 10% of familial breast cancer collectively, and additional moderate-risk genes such as CHEK2  and the recently reported BRIP1 (also called BACH1)  and PALB2 [8, 9] account for an even smaller percentage. This leaves a large proportion of the genetic basis of familial breast cancer unexplained.
Interestingly, BRCA2, p53, ATM, CHEK2, and BRIP1 all interact with the multifunctional tumour suppressor, BRCA1. BRCA1-interacting proteins are logical breast cancer candidates for two reasons. First, they are likely to be involved in some of the important roles of BRCA1 such as genome maintenance, transcription regulation, and cell cycle control and, if mutated, may result in the same highly penetrant and damaging effect as a BRCA1 mutation. Second, mutations in these interacting genes may prevent BRCA1 from performing vital functions, resulting in the same acute effect as a BRCA1 mutation itself. Recently, Pagan and colleagues  described the characterisation and functional analysis of a novel BRCA1-interacting protein, BCL6 corepressor-like 1 (BCoR-L1), that displays homology to several proteins involved in pathways such as DNA damage repair (BARD1 and Drosophila recombination repair protein 1) and transcription regulation (BCoR). Functional analysis thus far has revealed a role for BCoR-L1 in transcriptional corepression , placing it in a large group of proteins involved in the regulation of proliferation and apoptosis . It is well established that uncontrolled overexpression of oncogenes and repression or mutation of tumour suppressors contribute to tumourigenesis by disturbing these vitally important and tightly controlled cellular processes .
Evidence that BCoR-L1 operates primarily as a transcription corepressor includes its ability to dramatically reduce reporter gene expression through an interaction with the CtBP (carboxyl-terminal binding protein) corepressor via a PLDLS motif and the fact that it associates with a number of class II histone deacetylases (HDACs), factors also involved in transcription repression . Similarly, BRCA1 interacts with a number of proteins involved in chromatin remodelling and transcription control . Like BRCA1, BCoR-L1 is also involved in maintaining genomic stability after DNA damage.
In addition to its interaction with BRCA1, there is indirect evidence to suggest that BCoR-L1 may behave as a tumour suppressor. The BCoR-L1 protein appears to be expressed ubiquitously at low levels (including breast tissue), with high levels in reproductive tissues such as prostate and testes . However, BCoR-L1 expression was found to be decreased in a variety of breast cancer subjects, including BRCA1/2 mutation carriers and 'sporadic' breast cancer subjects . In addition, the BCoR-L1 gene is located at Xq26.1, a region reported to exhibit loss of heterozygosity (LOH) in many tumour types, including those of the breast [15–17]. BCoR-L1 is subject to complete X inactivation , and interestingly, an increased frequency of skewed X inactivation has been reported in both ovarian  and early-onset BRCA1/2 mutation-negative (BRCAX) breast [20, 21] cancer populations. It has been proposed that skewed X inactivation could provide a novel mechanism for nonrandom expression of a mutant tumour suppressor gene and thereby contribute to tumourigenesis.
We sought to determine whether there is a role for BCoR-L1 as a high-risk breast cancer predisposition gene by screening the coding region of the gene in 38 BRCAX breast cancer families by means of the highly sensitive mutation detection technique, denaturing high-performance liquid chromatography (DHPLC). The majority of families were chosen specifically for the presence of male breast cancer and/or early-onset prostate cancer, in combination with being a high-risk female breast cancer family, for two reasons. We hypothesised that an X-linked gene may be involved in these male cancer because males carry only one copy of the X chromosome and because BCoR-L1 has been found to be expressed highly in the prostate . A small number of breast cancer families in which the affected individuals showed haplotype sharing around the BCoR-L1 locus were also included in this study. In addition, we conducted quantitative real-time PCR (qRT-PCR) on lymphoblastoid cell lines (LCLs) from members of these families and a number of cancer cell lines to assess the role of BCoR-L1 expression in cancer and cancer families. We assessed BCoR-L1 expression in LCLs from breast cancer cases to determine whether BCoR-L1 expression was altered in familial breast cancer cases and, if so, whether this was associated with the presence of genetic variation. Analysis of X inactivation status of BCoR-L1 was also undertaken in order to assess the likely mode of inheritance of BCoR-L1 as a candidate tumour suppressor gene.
Materials and methods
X inactivation status analysis
X inactivation status was assessed by comparison of BCoR-L1 mRNA expression with human-specific primers (forward: CATATGATGTGACGGAATCTC; reverse: CCCTGGACTTTGTTGGGCA) in mouse-human or hamster-human hybrid cell lines containing a human active X chromosome (AHA-11aB1, A23-1aC1I5, t60-12, GM06318D, CHO-01416-M) or a human inactive X chromosome (LT23-1E2, t48-1a-1Daz4a, t11-4Aaz5, t75-2maz34-1a, t86-B1maz1b-3a, X8-6T2S1, CHO-01416-07). Comparison of expression levels between the two groups of cell lines (containing an inactive versus inactive human X chromosome) was used to establish whether BCoR-L1 is subject to X inactivation.
Multiple-case breast cancer families were ascertained through the Kathleen Cuningham Foundation Consortium for Research into Familial Breast Cancer (kConFab) . Inclusion criteria for all families in this study required that the family be classified as category 3 (high-risk) according to the National Breast Cancer Centre guidelines  and that the family not possess any known mutations in the BRCA1 or BRCA2 genes (BRCAX) at the time of initiation of the study. Ethics approvals were obtained from the ethics committees of the Peter MacCallum Cancer Institute (East Melbourne, Victoria, Australia) and the Queensland Institute of Medical Research (Brisbane, Queensland, Australia), and all subjects gave written informed consent. Male breast cancer families selected for study (n = 21) all contained one or more male breast cancer cases occurring on the same side of the family as at least three female breast cancer cases. A total of 11 male breast cancer cases and 16 female index cases (the youngest breast cancer case in the family from which biospecimens were available) were screened from these families. Prostate/female breast cancer families (n = 12) were chosen to contain at least one 'early-onset' prostate cancer case (cancer diagnosed at not more than 60 years of age) on the same side of the family as three or more female breast cancer cases. A total of 2 prostate cancer cases and 12 index female breast cancer cases were analysed from these families. Pedigrees were examined to ensure the absence of male-to-male transmission of the disease in these families because this would imply involvement of an autosomal gene. Additionally, families with female breast cancer only (n = 7) were selected for analysis because they demonstrated haplotype sharing in the same chromosomal region as BCoR-L1 (DXS1001, DXS1047, and DXS1227; LOD [logarithm of the odds] score greater than 0.5; data not shown) and are referred to as BCoR-L1 haplotype sharing families. Two BCoR-L1 variants were found in one family and were then genotyped in all family members from which biospecimens were available (n = 12). Mutation screening in the kConFab cohort during the course of the study subsequently identified BRCA2 mutation carriers in two male breast cancer families. Control subjects (46 females and 55 males) without cancer or a family history of cancer included subjects ascertained via the Queensland Blood Bank and a group of geriatric controls (average age of 80 years).
Screening for BCoR-L1variation
BCoR-L1 is expressed in two isoforms. The most common isoform is 1,711 amino acids in size and lacks exon 9, but the full-length protein (1,785 amino acids long) is derived from the alternative transcript. This study screened the entire coding region of the BCoR-L1 gene, including exon 9. Primers encompassing the 13 coding exons of BCoR-L1 (and surrounding intronic regions; GenBank: exons 2 to 8: Z82208; exons 9 to 14: AL136450) were designed using Primer3  (Table 1). Exon 4 was too large to be amplified at an optimal size for DHPLC analysis and was therefore analysed with 10 overlapping polymerase chain reaction (PCR) fragments. 'Standard' PCR reactions were carried out in a 20-μl mixture containing 15 ng of genomic DNA, and a final concentration of 20 pmol of each primer, 200 μM each of dATP, dCTP, dGTP, and dTTP (Promega Corporation, Madison, WI, USA), 1.5 mM MgCl2, 1× PCR buffer, and 1 U AmpliTaq Gold polymerase (PE Applied Biosystems, Foster City, CA, USA). Any variation to the reaction is detailed in Table 1, along with a description of 'touchdown' PCR amplification conditions. All products (and H2O controls) were visualised on a 1.5% agarose gel. Male samples were mixed with sequence-confirmed wild-type female PCR product (2:1) to encourage heteroduplexes to form for successful DHPLC analysis. Samples were denatured by heating to 95°C for 5 minutes and cooling to 60°C over a period of 30 minutes and then analysed on a Varian Helix DHPLC system (Varian, Inc., Palo Alto, CA, USA) at the recommended melt temperature(s) as determined by the Stanford DHPLC Melt program  (Table 1). Analysis of results was carried out using Star Workstation Reviewer software (version 5, Varian) and any aberrant or shifted profiles were reamplified for confirmation of the aberrant profile by repeat DHPLC before being sequenced using the Big-Dye (version 3.1) sequencing chemistry and PE Applied Biosystems 377 sequencer.
Loss of heterozygosity analysis
LOH analysis was carried out on tumour blocks from the BCoR-L1 haplotype sharing family carrying the exon 4 c.516T>C variant, because genotyping analysis revealed that the variant segregated with breast cancer. Macrodissected tumour and adjacent cancer-uninvolved tissue DNA was extracted from tumour blocks by means of a modified version of the method of Levi and colleagues , and 2 μl of each DNA (plus 20 ng of lymphocyte-derived germline DNA from the same subject) was then added to separate 20-μl PCR reactions, as detailed above. Primers used were (forward) TCAACACCCAAATGAGCAAA and (reverse) GAACAGAGTGGGGCACAGAG to give a product of 242 base pairs. 'Touchdown' PCR was used with an annealing temperature of 50°C. PCR products were then purified (Qiagen Inc., Valencia, CA, USA) and sequenced. LOH was evaluated by scoring the absence of the allele in the sequencing trace of the tumour, compared to matching germline DNA.
All LCLs and normal and cancer cell lines were grown in RPMI 1640 media with 10% fetal calf serum and 1% penicillin/streptomycin. Cell lines used are detailed in Figure 1a. RNA was extracted from LCLs and cell lines using TriReagent (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer's instructions, and 1 μg of each sample was placed in a reverse transcription-PCR reaction using Superscript III RNAse H- Reverse Transcriptase as directed by the manufacturer (Invitrogen Corporation, Carlsbad, CA, USA). qRT-PCR was then carried out in a 15 μl reaction containing a final volume of 20 ng of cDNA, 20 pmol of each primer, and 7.5 μl of Platinum SYBR Green qPCR Supermix UDG (Invitrogen Corporation). Primer sequences used are detailed in Table 2. qRT-PCR conditions were 50°C for 2 minutes and 95°C for 2 minutes, 40 cycles of 95°C for 20 seconds, 60°C for 15 seconds, and 72°C for 20 seconds (acquiring) on a Rotor-Gene RG-3000 Real-Time PCR machine (Corbett Research Australia, Mortlake, New South Wales, Australia). All samples were run in duplicate and were repeated if profiles did not replicate according to Rotor-Gene analysis software (version 5). Accordingly, the maximum standard deviation allowed for a pair of duplicates ('Rep. Ct Std. Dev' in analysis software) was low (≤0.2). Expression levels were presented as the mean of two duplicates, normalised to expression of either GAPDH (all samples) or DIDO1 (death inducer-obliterator 1) (LCLs only). Expression levels of different groups were compared using the Student t test (two-tailed).
Assessment of expression controls for lymphoblastoid cell lines
RT-PCR expression controls are typically chosen for their stability of expression not only during various phases of the cell cycle, but also between different tissue types. However, it is well known that these characteristics are very difficult to obtain. It has been reported that widely accepted expression controls such as β-actin and GAPDH show unacceptable variation in expression in a large number of tissues and are therefore not ideal controls . We sought to find a suitable expression control for analysis of expression in LCLs and compared this with two widely used expression controls, GAPDH and ubiquitin C (UBC). Cheung and colleagues  used microarray analysis to establish the variability of expression of 5,184 genes in LCLs taken from random individuals. We evaluated the 100 least variably expressed genes for suitability as an LCL expression control for our project via a PubMed  search of the literature to identify the genes that (a) had not been reported to be associated with cancer of any kind and (b) were not in a region of LOH or linkage to any cancer. DIDO1 was chosen according to these criteria.
Results and Discussion
BCoR-L1 was selected as an interesting breast cancer candidate gene for a number of reasons. BCoR-L1 interacts with the important breast cancer susceptibility gene product, BRCA1, and there is the possibility that BCoR-L1 is involved, with BRCA1, in critical DNA repair and cell growth pathways. Indeed, mutations in repressor proteins have been implicated in several diseases, including cancer . For example, functional mutations in the transcriptional repressor Rb gene result in dysregulation of cell cycle control . In addition, the BCoR-L1 gene is located at Xq26.1, a region that exhibits LOH in cancers, including breast cancer, and there is some evidence that BCoR-L1 mRNA expression is deregulated in breast cancer. Skewed X inactivation has been reported in breast and ovarian cancer subjects, indicating the possible presence of an X-linked cancer predisposition gene.
X inactivation status analysis
Analysis of the hybrid cell lines showed that BCoR-L1 was not expressed in 4/5 hybrid cell lines containing an inactive X chromosome and was expressed at very low levels in the fifth cell line t48-1a-1Daz4a, using hybrids containing an active X chromosome as reference. These results indicate that BCoR-L1 is subject to X chromosome inactivation in humans, which is in agreement with the study of Carrel and Willard , who showed that the proximal UTP14A gene was expressed in 3/9 hybrids whereas BCOR-L1 (FLJ11362) was silent in all 9. As some of the hybrids in this study overlap, BCOR-L1 is silenced in 11/11 hybrids studied to date. The fact that BCoR-L1 is subject to X inactivation suggests that only a single mutational event in the BCoR-L1 gene would be required to initiate tumourigenesis.
Variation in the BCoR-L1gene
DHPLC analysis of the coding region of BCoR-L1 in 48 members of 38 high-risk BRCAX breast cancer families revealed only four different sequence variations (Table 3). A nucleotide variation in exon 4 (c.516T>C; p.N172N) was found in one breast cancer family known to share a haplotype at the BCoR-L1 locus. This variant was carried primarily by breast cancer cases from this family (Figure 2). However, this c.516T>C nucleotide substitution is not likely to be functional because it does not result in an amino acid change and is not predicted by in silico modelling to have any effect on mRNA structure (MFOLD ) and the wild-type T allele is not conserved in either mouse or rat (PipMaker ). Investigation of exonic splice enhancer (ESE) sites by means of ESEfinder (version 2.0)  revealed that the variant allele produces an SC35 site (of value 2.455), but this score is very close to the SC35 threshold of 2.383. Similar analysis using Rescue ESE  revealed no change. Additionally, analysis of codon usage of the AAT codon (wild-type) in the human genome versus the AAC codon (variant) showed that the two codons are used at similar frequencies (16.8 versus 19.1/1,000 codons) . Finally, LOH analysis of the c.516T>C variant gave no evidence of the change being involved in tumourigenesis. LOH analysis of tumour blocks and germline DNA from all four breast cancer cases in this family revealed either loss of the variant allele or no LOH (data not shown), indicating that this variant is unlikely to be pathogenic.
The intron 5 c.3608-156C>T variant was found only in members of the family who carried the exon 4 synonymous variant p.N172N (Figure 2). Due to its deeply intronic location, c.3608-156C>T is not predicted to have any functional effect. SpliceSiteFinder did not predict any changes to splicing as a result of this nucleotide substitution . Furthermore, mRNA expression analysis (see below) of BCoR-L1 in two of the variant-carrying breast cancer cases and one wild-type non-breast cancer subject from this family revealed no evidence for consistent differences in expression in LCLs. Relative to the unaffected wild-type family control, one variant carrier displayed increased expression and the other displayed decreased expression (Figure 3a,b; subjects marked with #).
The two other variants detected in and around the BCoR-L1 coding region in this study, c.625G>A (p.G209S) and c.5075+21C>T, were found in similar frequencies in the control sample. Similarly, there were no major differences between groups when the study sample was divided into male breast, prostate, or BCoR-L1 haplotype sharing families. Although the exon 4 p.G209S variant is a missense amino acid substitution, this change is predicted by SIFT (Sorting Intolerant From Tolerant) to be 'tolerated' . p.G209S is also located in a region of BCoR-L1 that is not thought to be involved in BRCA1 interaction or transcription repression . Additionally, qRT-PCR analysis of BCoR-L1 expression in breast cancer cases carrying the p.G209S and c.5075+21C>T variants showed no differences when compared with controls (Figure 3c,d), with overlapping standard deviations for expression. Furthermore, both variants were present in individuals found to carry pathogenic BRCA2 mutations during the course of the study.
Overall, we detected very little variation in the coding region (and surrounding intronic region) of the BCoR-L1 gene in our population of familial cancer cases. The low level of variation detected in the BCoR-L1 gene is consistent with reports that the X chromosome carries very little variation when compared with autosomal chromosomes [39–41].
To further investigate the role of BCoR-L1 in familial breast cancer and male cancers, we undertook qRT-PCR expression analysis on LCLs from subjects previously screened for BCoR-L1 coding region variation. Alteration of BCoR-L1 expression in LCLs from breast cancer-affected family members may indicate the presence of a regulatory mutation in a noncoding region of the gene. In addition, since various HDACs are aberrantly expressed in a number of cancers susceptible to treatment using HDAC inhibitors , including breast cancer , we speculated that BCoR-L1, given its association with HDACs, might also be abnormally expressed in breast cancer cases. We thus assessed BCoR-L1 expression in a range of breast, ovarian, and prostate cancer lines.
DIDO1expression in lymphoblastoid cell lines and cell lines
To investigate the suitability of DIDO1 as an LCL expression control, we analysed expression of DIDO1, GAPDH, and UBC in all LCLs and cell lines tested (Figure 4a,b). Interestingly, we found that DIDO1 expression varies considerably less in LCLs than GAPDH or UBC, with GAPDH and UBC showing similar levels of expression variation (Figure 4a). The interquartile range for DIDO1 was 2.5-fold and 2.7-fold less than those for UBC and GAPDH, respectively. In cancer cell lines, all three expression control genes showed large ranges of expression levels across lines, with GAPDH perhaps showing the least variation (Figure 4b). Therefore, DIDO1 is an improved expression control for LCLs but has variability similar to the commonly used GAPDH control in normal and cancer cell lines.
BCoR-L1expression in lymphoblastoid cell lines from breast cancer families
Twenty-nine LCLs from breast cancer cases and their family members (23 families in total, including 14 with male breast cancer, 7 with prostate cancer, and 2 BCoR-L1 haplotype sharing families) were analysed for changes in BCoR-L1 expression when compared with LCLs from 6 healthy controls (Figure 3a–d). Although expression of BCoR-L1 appeared to be greatly variable, there were no apparent differences in expression levels between breast cancer cases and controls, nor were there any differences between groups when segregated by family cancer type (that is, male breast, prostate, and so on). Likewise, there was no indication of any association between BCoR-L1 genotype and expression. Skewed X chromosome inactivation data were available for a limited number of samples (n = 8). However, skewing did not correlate with BCoR-L1 expression or genotype (data not shown). Results correlating expression with sample source or sample genotype were similar using GAPDH (Figure 3a,c) or DIDO1 (Figure 3b,d) normalisation, although the decreased variability observed in normal controls for DIDO1 normalisation supported our earlier observations that DIDO1 is an improved control for LCL expression analysis. The standard deviation of expression in control LCLs was approximately 20% less for DIDO1 compared with GAPDH. Overall, it appears that BCoR-L1 expression is not altered in familial breast cancer cases, even for subgroups defined by male cancer type, and it is unlikely that there is any variation in the BCoR-L1 gene (detected or otherwise) which has a profound effect on expression.
BCoR-L1expression in cancer and normal cell lines
To assess a possible role for BCoR-L1 in tumourigenesis, we also analysed BCoR-L1 expression in various ovarian, breast, and prostate cancer and normal cell lines (Figure 1a,b). Most normal and cancer cell lines exhibited increased expression levels compared with LCLs. Once again, it was observed that BCoR-L1 expression is highly variable in both cancer and normal cell lines, with up to 13-fold differences in expression observed. There were no significant differences between the mean BCoR-L1 expression in normal cell lines compared with cancer cell lines, but individual ovarian and breast cancer cell lines showed significantly increased expression compared with the mean expression in normal cell lines. Markedly elevated levels of BCoR-L1 (P < 0.05) were observed for a total of 4/10 ovarian cancer cell lines (OVCAR3, SKOV3, A2780, 27/87; 4-fold to 13-fold upregulation compared with the HOSE17.1 normal ovarian epithelial control) and 2/13 breast cancer cell lines (BT20 and T47D; 3-fold and 4-fold upregulation compared with SVCT normal breast control). This was interesting, considering that skewed X inactivation has been reported in ovarian cancer cases . It would also seem to suggest that dysregulation of expression in the form of upregulation may play a role in tumourigenesis. However, a study using a BCoR-L1-containing Affymetrix microarray (Affymetrix, Santa Clara, CA, USA) did not provide any evidence for BCoR-L1 deregulation in ovarian cancers . Comparison of gene expression between 37 advanced-stage serous ovarian carcinomas and normal ovarian surface epithelium cytobrushings revealed that BCoR-L1 was not one of 1,191 genes that were significantly differentially regulated (defined as greater than or equal to 1.5-fold change in expression). This contrasts with the 4- to 13-fold increased expression we observed for specific cancer lines compared with normal ovarian epithelial cell lines.
There was no evidence that the hormone receptor status of each cell line correlated with these BCoR-L1 expression differences because the highly estrogen receptor (ER)- and progesterone receptor (PR)-positive breast cancer cell line T47D showed similar BCoR-L1 expression to BT20, a breast cancer cell line that does not express ER or PR . In addition, the ovarian cancer cell lines OVCAR3 (ER-positive) and SKOV3 (ER-negative) expressed similar levels of BCoR-L1 . Investigation of the X chromosome karyotype of these ovarian cancer cell lines did reveal a possible relationship with BCoR-L1 expression. Karyotype data were available for OVCAR3, SKOV3, OVCAR5, and OVCAR8 , with the BCoR-L1-overexpressing lines OVCAR3 and SKOV3 found to possess three copies of Xq and four X chromosomes, respectively. Conversely, OVCAR5 and OVCAR8 each possess only one X chromosome and express normal levels of BCoR-L1. Information on the X chromosome karyotype of breast cancer cell lines was limited, but there was no indication of an association between karyotype and BCoR-L1 expression [47, 48]. The finding that BCoR-L1 expression tends to correlate with supernumary X chromosomes suggests that this overexpression probably does not contribute to carcinogenesis but may occur as a result of carcinogenesis. Although it is unknown whether the superfluous X chromosomes in OVCAR3 and SKOV3 are active, it has been reported that ovarian (and breast) tumours can possess multiple active X chromosomes [48–51].
The aim of the present study was to attempt to elucidate a role for BCoR-L1 as a high-risk breast cancer predisposition gene by mutation screening of well-characterised non-BRCA1/2 familial breast cancer subjects who were selected to maximise the probability of identifying a mutation. We detected minimal variation in the coding region of BCoR-L1, which may imply the importance of maintaining the structural integrity of the BCoR-L1 protein. It is also unlikely that noncoding region variation in the BCoR-L1 gene is involved in breast cancer predisposition as we did not detect any significant changes in expression between cancer cases and cell lines and controls. The absence of pathogenic coding mutations or expression deregulation in this set of familial cases indicates that BCoR-L1 is extremely unlikely to be a major high-risk familial breast cancer predisposition gene. However, it is still possible that BCoR-L1 could be involved in breast cancer predisposition as a moderate- or low-penetrance risk gene, as has been found with CHEK2 , BRIP1 , and PALB2 [8, 9] in large studies of BRCAX cases and controls. The involvement of BCoR-L1 in ovarian cancer may also be worthy of investigation, and further functional analysis of the BCoR-L1 protein will help to elucidate the involvement of BCoR-L1 in various essential pathways.
BCL6 corepressor-like 1
- BRCAX :
denaturing high-performance liquid chromatography
- DIDO1 :
death inducer-obliterator 1
exonic splice enhancer
- GAPDH :
Kathleen Cuningham Foundation Consortium for Research into Familial Breast Cancer
lymphoblastoid cell line
loss of heterozygosity
polymerase chain reaction
quantitative real-time polymerase chain reaction
- UBC :
Peto J, Collins N, Barfoot R, Seal S, Warren W, Rahman N, Easton DF, Evans C, Deacon J, Stratton MR: Prevalence of BRCA1 and BRCA2 gene mutations in patients with early-onset breast cancer. J Natl Cancer Inst. 1999, 91: 943-949. 10.1093/jnci/91.11.943.
Ford D, Easton DF, Stratton M, Narod S, Goldgar D, Devilee P, Bishop DT, Weber B, Lenoir G, Chang-Claude J, et al: Genetic heterogeneity and penetrance analysis of the BRCA1 and BRCA2 genes in breast cancer families. The Breast Cancer Linkage Consortium. Am J Hum Genet. 1998, 62: 676-689. 10.1086/301749.
Sidransky D, Tokino T, Helzlsouer K, Zehnbauer B, Rausch G, Shelton B, Prestigiacomo L, Vogelstein B, Davidson N: Inherited p53 gene mutations in breast cancer. Cancer Res. 1992, 52: 2984-2986.
Chenevix-Trench G, Spurdle AB, Gatei M, Kelly H, Marsh A, Chen X, Donn K, Cummings M, Nyholt D, Jenkins MA, et al: Dominant negative ATM mutations in breast cancer families. J Natl Cancer Inst. 2002, 94: 205-215.
Lynch ED, Ostermeyer EA, Lee MK, Arena JF, Ji H, Dann J, Swisshelm K, Suchard D, MacLeod PM, Kvinnsland S, et al: Inherited mutations in PTEN that are associated with breast cancer, cowden disease, and juvenile polyposis. Am J Hum Genet. 1997, 61: 1254-1260. 10.1086/301639.
Bernstein JL, Teraoka SN, John EM, Andrulis IL, Knight JA, Lapinski R, Olson ER, Wolitzer AL, Seminara D, Whittemore AS, et al: The CHEK2*1100delC allelic variant and risk of breast cancer: screening results from the Breast Cancer Family Registry. Cancer Epidemiol Biomarkers Prev. 2006, 15: 348-352. 10.1158/1055-9965.EPI-05-0557.
Seal S, Thompson D, Renwick A, Elliott A, Kelly P, Barfoot R, Chagtai T, Jayatilake H, Ahmed M, Spanova K, et al: Truncating mutations in the Fanconi anemia J gene BRIP1 are low-penetrance breast cancer susceptibility alleles. Nat Genet. 2006, 38: 1239-1241. 10.1038/ng1902.
Erkko H, Xia B, Nikkila J, Schleutker J, Syrjakoski K, Mannermaa A, Kallioniemi A, Pylkas K, Karppinen SM, Rapakko K, et al: A recurrent mutation in PALB2 in Finnish cancer families. Nature. 2007, 446: 316-319. 10.1038/nature05609.
Rahman N, Seal S, Thompson D, Kelly P, Renwick A, Elliott A, Reid S, Spanova K, Barfoot R, Chagtai T, et al: PALB2, which encodes a BRCA2-interacting protein, is a breast cancer susceptibility gene. Nat Genet. 2007, 39: 165-167. 10.1038/ng1959.
Pagan JK, Arnold J, Hanchard KJ, Kumar R, Bruno T, Jones MJ, Richard DJ, Forrest A, Spurdle A, Verdin E, et al: A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP. J Biol Chem. 2007, 282: 15248-15257. 10.1074/jbc.M700246200.
Jepsen K, Rosenfeld MG: Biological roles and mechanistic actions of co-repressor complexes. J Cell Sci. 2002, 115 (Pt 4): 689-698.
Hanahan D, Weinberg RA: The hallmarks of cancer. Cell. 2000, 100: 57-70. 10.1016/S0092-8674(00)81683-9.
Rosen EM, Fan S, Pestell RG, Goldberg ID: BRCA1 gene in breast cancer. J Cell Physiol. 2003, 196: 19-41. 10.1002/jcp.10257.
van 't Veer LJ, Dai H, van de Vijver MJ, He YD, Hart AA, Mao M, Peterse HL, van der Kooy K, Marton MJ, Witteveen AT, et al: Gene expression profiling predicts clinical outcome of breast cancer. Nature. 2002, 415: 530-536. 10.1038/415530a.
Loupart ML, Adams S, Armour JA, Walker R, Brammar W, Varley J: Loss of heterozygosity on the X chromosome in human breast cancer. Genes Chromosomes Cancer. 1995, 13: 229-238. 10.1002/gcc.2870130402.
Choi C, Cho S, Horikawa I, Berchuck A, Wang N, Cedrone E, Jhung SW, Lee JB, Kerr J, Chenevix-Trench G, et al: Loss of heterozygosity at chromosome segment Xq25-26.1 in advanced human ovarian carcinomas. Genes Chromosomes Cancer. 1997, 20: 234-242. 10.1002/(SICI)1098-2264(199711)20:3<234::AID-GCC3>3.0.CO;2-3.
Choi C, Kim MH, Juhng SW: Loss of heterozygosity on chromosome XP22.2-p22.13 and Xq26.1-q27.1 in human breast carcinomas. J Korean Med Sci. 1998, 13: 311-316.
Carrel L, Willard HF: X-inactivation profile reveals extensive variability in X-linked gene expression in females. Nature. 2005, 434: 400-404. 10.1038/nature03479.
Buller RE, Sood AK, Lallas T, Buekers T, Skilling JS: Association between nonrandom X-chromosome inactivation and BRCA1 mutation in germline DNA of patients with ovarian cancer. J Natl Cancer Inst. 1999, 91: 339-346. 10.1093/jnci/91.4.339.
Kristiansen M, Langerod A, Knudsen GP, Weber BL, Borresen-Dale AL, Orstavik KH: High frequency of skewed X inactivation in young breast cancer patients. J Med Genet. 2002, 39: 30-33. 10.1136/jmg.39.1.30.
Kristiansen M, Knudsen GP, Maguire P, Margolin S, Pedersen J, Lindblom A, Orstavik KH: High incidence of skewed X chromosome inactivation in young patients with familial non-BRCA1/BRCA2 breast cancer. J Med Genet. 2005, 42: 877-880. 10.1136/jmg.2005.032433.
Mann GJ, Thorne H, Balleine RL, Butow PN, Clarke CL, Edkins E, Evans GM, Fereday S, Haan E, Gattas M, et al: Analysis of cancer risk and BRCA1 and BRCA2 mutation prevalence in the kConFab familial breast cancer resource. Breast Cancer Res. 2006, 8: R12-10.1186/bcr1377.
National Breast Cancer Centre: Advice about familial aspects of breast cancer and epithelial ovarian cancer: a guide for health professionals. [http://www.nbcc.org.au/bestpractice/resources/BOG182_adviceaboutfamiliala.pdf]
Rozen S, Skaletsky HJ: Primer3 on the WWW for general users and for biologist programmers. Bioinformatics: Methods and Protocols (Methods in Molecular Biology). Edited by: Krawetz S, Misener S. 2000, Totowa, NJ: Humana Press Inc, 365-386. [http://frodo.wi.mit.edu/primer3/input.htm]
Jones AC, Austin J, Hansen N, Hoogendoorn B, Oefner PJ, Cheadle JP, O'Donovan MC: Optimal temperature selection for mutation detection by denaturing HPLC and comparison to single-stranded conformation polymorphism and heteroduplex analysis. Clin Chem. 1999, 45 (8 Pt 1): 1133-1140.
Levi S, Urbano-Ispizua A, Gill R, Thomas DM, Gilbertson J, Foster C, Marshall CJ: Multiple K-ras codon 12 mutations in cholangiocarcinomas demonstrated with a sensitive polymerase chain reaction technique. Cancer Res. 1991, 51: 3497-3502.
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3: RESEARCH0034-10.1186/gb-2002-3-7-research0034.
Cheung VG, Conlin LK, Weber TM, Arcaro M, Jen KY, Morley M, Spielman RS: Natural variation in human gene expression assessed in lymphoblastoid cells. Nat Genet. 2003, 33: 422-425. 10.1038/ng1094.
Gabellini D, Green MR, Tupler R: When enough is enough: genetic diseases associated with transcriptional derepression. Curr Opin Genet Dev. 2004, 14: 301-307. 10.1016/j.gde.2004.04.010.
Thiel G, Lietz M, Hohl M: How mammalian transcriptional repressors work. Eur J Biochem. 2004, 271: 2855-2862. 10.1111/j.1432-1033.2004.04174.x.
Zuker M: Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 2003, 31: 3406-3415. 10.1093/nar/gkg595.
Schwartz S, Zhang Z, Frazer KA, Smit A, Riemer C, Bouck J, Gibbs R, Hardison R, Miller W: PipMaker – a web server for aligning two genomic DNA sequences. Genome Res. 2000, 10: 577-586. 10.1101/gr.10.4.577.
Cartegni L, Wang J, Zhu Z, Zhang MQ, Krainer AR: ESEfinder: a web resource to identify exonic splicing enhancers. Nucleic Acids Res. 2003, 31: 3568-3571. 10.1093/nar/gkg616.
Fairbrother WG, Yeh RF, Sharp PA, Burge CB: Predictive identification of exonic splicing enhancers in human genes. Science. 2002, 297: 1007-1013. 10.1126/science.1073774.
Nakamura Y, Gojobori T, Ikemura T: Codon usage tabulated from international DNA sequence databases: status for the year 2000. Nucleic Acids Res. 2000, 28: 292-10.1093/nar/28.1.292.
Alex Dong Li's SpliceSiteFinder. [http://www.genet.sickkids.on.ca/~ali/splicesitefinder.html]
Ng PC, Henikoff S: Predicting deleterious amino acid substitutions. Genome Res. 2001, 11: 863-874. 10.1101/gr.176601.
Ross MT, Grafham DV, Coffey AJ, Scherer S, McLay K, Muzny D, Platzer M, Howell GR, Burrows C, Bird CP, et al: The DNA sequence of the human X chromosome. Nature. 2005, 434: 325-337. 10.1038/nature03440.
Tsui C, Coleman LE, Griffith JL, Bennett EA, Goodson SG, Scott JD, Pittard WS, Devine SE: Single nucleotide polymorphisms (SNPs) that map to gaps in the human SNP map. Nucleic Acids Res. 2003, 31: 4910-4916. 10.1093/nar/gkg664.
International HapMap Consortium: A haplotype map of the human genome. Nature. 2005, 437: 1299-1320. 10.1038/nature04226.
Johnstone RW: Histone-deacetylase inhibitors: novel drugs for the treatment of cancer. Nat Rev Drug Discov. 2002, 1: 287-299. 10.1038/nrd772.
Kawai H, Li H, Avraham S, Jiang S, Avraham HK: Overexpression of histone deacetylase HDAC1 modulates breast cancer progression by negative regulation of estrogen receptor alpha. Int J Cancer. 2003, 107: 353-358. 10.1002/ijc.11403.
Donninger H, Bonome T, Radonovich M, Pise-Masison CA, Brady J, Shih JH, Barrett JC, Birrer MJ: Whole genome expression profiling of advance stage papillary serous ovarian cancer reveals activated pathways. Oncogene. 2004, 23: 8065-8077. 10.1038/sj.onc.1207959.
Walsh MD, Luckie SM, Cummings MC, Antalis TM, McGuckin MA: Heterogeneity of MUC1 expression by human breast carcinoma cell lines in vivo and in vitro. Breast Cancer Res Treat. 1999, 58: 255-266. 10.1023/A:1006345301364.
Treeck O, Haldar C, Ortmann O: Antiestrogens modulate MT1 melatonin receptor expression in breast and ovarian cancer cell lines. Oncol Rep. 2006, 15: 231-235.
Roschke AV, Tonon G, Gehlhaus KS, McTyre N, Bussey KJ, Lababidi S, Scudiero DA, Weinstein JN, Kirsch IR: Karyotypic complexity of the NCI-60 drug-screening panel. Cancer Res. 2003, 63: 8634-8647.
Sirchia SM, Ramoscelli L, Grati FR, Barbera F, Coradini D, Rossella F, Porta G, Lesma E, Ruggeri A, Radice P, et al: Loss of the inactive X chromosome and replication of the active X in BRCA1-defective and wild-type breast cancer cells. Cancer Res. 2005, 65: 2139-2146. 10.1158/0008-5472.CAN-04-3465.
Kimmel VM: Clinical-cytological correlations of mammary carcinoma based upon sex-chromatin counts. Cancer. 1957, 10: 922-927. 10.1002/1097-0142(195709/10)10:5<922::AID-CNCR2820100509>3.0.CO;2-7.
Wang N, Cedrone E, Skuse GR, Insel R, Dry J: Two identical active X chromosomes in human mammary carcinoma cells. Cancer Genet Cytogenet. 1990, 46: 271-280. 10.1016/0165-4608(90)90112-N.
Benoit MH, Hudson TJ, Maire G, Squire JA, Arcand SL, Provencher D, Mes-Masson AM, Tonin PN: Global analysis of chromosome X gene expression in primary cultures of normal ovarian surface epithelial cells and epithelial ovarian cancer cell lines. Int J Oncol. 2007, 30: 5-17.
The authors thank Heather Thorne, Eveline Niedermayr, Lynda Williams, Danni Surace, and all the kConFab research nurses and staff, the heads and staff of the Family Cancer Clinics, and the Clinical Follow Up Study (funded by National Health and Medical Research Council [NHMRC] grants 145684 and 288704) for their contributions to this resource and the many families who contribute to kConFab. kConFab is supported by grants from the National Breast Cancer Foundation and the NHMRC and by the Queensland Cancer Fund, the Cancer Councils of New South Wales, Victoria, Tasmania, and South Australia, and the Cancer Foundation of Western Australia. The authors thank Helene Holland for supplying data and Anna Marsh for technical advice and assistance on DHPLC. Judith Clements and Ying Dong supplied prostate cancer cell lines used in this study, and Vivien G Cheung kindly made available unpublished data on mRNA expression levels in LCLs. This research was supported by grants from the Queensland Cancer Fund and National Breast Cancer Foundation. GC-T and KKK are NHMRC Principal Research Fellows, and ABS is funded by an NHMRC Career Development Award.
The authors declare that they have no competing interests.
FL carried out the screening of the gene and the LOH and expression analysis and drafted the manuscript. JA provided RNA from all of the breast and ovarian cell lines. GJM and GMP performed haplotype sharing analysis. CJB performed the X inactivation status analysis and drafted the relevant section of the manuscript. DBY contributed to the experimental study design and provided sequence data on BCoR-L1. kConFab recruited and collected subjects for the study. GC-T and KKK participated in the design and coordination of the study. ABS conceived the study, participated in its design and coordination, and helped to draft the manuscript. All authors read and approved the final manuscript.
An erratum to this article is available at http://dx.doi.org/10.1186/bcr2153.
About this article
Cite this article
Lose, F., Arnold, J., Young, D.B. et al. BCoR-L1 variation and breast cancer. Breast Cancer Res 9, R54 (2007). https://doi.org/10.1186/bcr1759