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  • Meeting abstract
  • Open Access

REAL Real-Time!

  • 1,
  • 1 and
  • 1
Breast Cancer Research20002 (Suppl 1) :P8.02

https://doi.org/10.1186/bcr174

  • Published:

Keywords

  • Dilution Series
  • Gene Expression Analysis
  • Dwell Time
  • Full Text
  • Detection Strategy

Full text

Recent advances in real-time PCR analysis have led to significant improvements in the accuracy and simplicity of DNA quantification and gene expression analysis. Here we demonstrate the new Bio-Rad iCycler iQ system capabilities. When PCR is conducted on 96 replicates, we achieve a uniformity with a CV of less than 1%, consistent with that of other well-known systems for real-time PCR analysis. We demonstrate the ability to distinguish a two-fold dilution series of human genomic DNA down to 125 genomic equivalents. We also show a wide dynamic range over which quantification is possible, beginning with plasmids or genomic DNA. The iCycler iQ system is designed to work with many detection strategies; here we show the iCycler iQs' ability to employ a variety of techniques, including SYBR Green I, TaqMan® and Molecular Beacons. Finally, the iCycler iQ's unique ability to analyse data at any point within a cycle or dwell time can be a significant advantage when evaluating certain detection chemistries including molecular beacons.

Authors’ Affiliations

(1)
2000 Alfred Nobel Drive, Hercules, CA 94547, USA

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