Volume 8 Supplement 2
Poly(ADPribosyl)ation of CTCF: role in breast tumourigenesis
© BioMed Central Ltd 2006
Published: 01 November 2006
CTCF is a conserved, ubiquitous and multifunctional 11 Zn finger (ZF) transcription factor with features of a tumour suppressor. CTCF regulates transcription in diverse modes, such as promoter activation and repression, silencing, constitutive and methylation-dependent chromatin insulation. We have previously reported that CTCF can be post-translationally regulated by poly(ADPribosyl)ation and that this modification modulates the insulator function of CTCF [1, 2]. The purpose of the present study is to investigate the role of CTCF poly(ADPribosyl)ation in normal and breast cancer cells.
The following techniques have been used in this investigation: western analysis, mass spectrometry, immunoprecipitation, cell cultures, transient transfection, primary cultures from normal and tumour tissues, cellular fractionation and laser capture microdissection.
This research addresses the molecular mechanisms of breast tumourigenesis: CTCF180 and CTCF130 may regulate different sets of genes and/or different cell functions specific for normal and cancer cells, respectively. The loss of CTCF poly(ADPribosyl)ation could also lead to epigenetic disturbances. Our data obtained so far indicate that the transition from CTCF180 to CTCF130 could be a hallmark of tumour development. We envisage the potential use of both CTCF isoforms as biological markers for breast tumourigenesis.
This work was funded by Breast Cancer Campaign, The Medical Research Council, and The University of Essex.
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