Fig. 5

AMD1 increases eIF5A hypusination and TCF4 protein level. (A) Spermidine induces eIF5A hypusination that contributes to its activation. (B) Expression of TCF4, AMD1, eIF5A and eIF5A hypusination was examined by Western blotting in BT549 and Hs578T cells treated with or without spermidine (30ng/ml) for a period of 0, 12–24 h. (C, D) Expression of TCF4, AMD1, eIF5A and eIF5A hypusination was examined by Western blotting in MDA-MB468 and SUM159 cells with stable empty vector or knockdown of AMD1 expression (C) as well as BT549 and Hs578T cells with stable empty vector or ADM1 expression (D). (E, F) Expression of TCF4, AMD1, eIF5A and eIF5A hypusination was examined by Western blotting in MDA-MB468 (E) and SUM159 (F) cells with stable empty vector or knockdown of AMD1 expression following treatment with or without spermidine (30ng/ml) for a period of 24 h