Fig. 2

AMD1 overexpression correlated with its copy number amplification and promoter hypomethylation. (A, B) Box-plots indicated the correlation of AMD1 mRNA expression with its copy number variants status (gain, diploid and deletion) in breast cancer from MEBTABRIC dataset (A) and TCGA dataset (B). (C, D) Box-plots showed the association of AMD1 mRNA level with copy number variants (gain, diploid and deletion) in different subtypes of breast cancer from MEBTABRIC dataset (C) and TCGA dataset (D). (E, F) Analysis of the proportion of AMD1’s copy number status (gain or no gain) in different subtypes of breast cancer from MEBTABRIC dataset (E) and TCGA dataset (F). (G) Box-plots indicated AMD1 promoter methylation in different subtypes of breast cancer using multiple 450 K probes (TGCA dataset). (H) AMD1 promoter methylation was analyzed in breast cancer cell lines by Methylation-specific PCR.