Fig. 1

Elevated AMD1 expression was tightly correlated with BLBC. (A) Box-plots indicated AMD1 mRNA expression in four different subtypes of breast cancer from six datasets (TCGA, METABRIC, GSE25066, NKI295, GSE7390 and GSE22358). (B) Box-plots indicated AMD1 mRNA expression in BLBC and luminal cell lines from two different datasets (E-TAMB-181 and E-TAMB-157). Comparisons are made using the two-tailed Student’s t-test. (C, D) Expression of AMD1 mRNA was analyzed by quantitative real-time PCR in breast cancer cell lines (C), and the comparison between basal-like and luminal breast cancer cell lines from (C) was analyzed (D). Data are shown as mean ± SD based on three independent experiments. (E) Expression of AMD1 in cells from (C) was examined by western blotting