Fig. 7

SNORA68 regulates the U2AF2/RPL23/c-Myc axis in vivo. A Average tumor volumes of xenograft mice were measured every three days. B, C Subcutaneous tumors and tumor weights are shown. D The expression of stemness markers (Nanog, OCT4 and SOX2) in the four groups was verified by Western blot. E Western blot was used to determine RPL23, U2AF2 and c-Myc expression in each group. F The expression of RPL23 in the nucleoplasm and nucleolus in the four groups of mice was measured by Western blot. G ISH and IHC were used to determine SNORA68 and ki67 expression. H Schematic of the mechanisms by which SNORA68-U2AF2 promotes TNBC stemness by retaining RPL23 in the nucleolus and reducing the RPL23-mediated decrease in c-Myc expression in the nucleoplasm. The data are presented as the mean ± SD of three independent experiments performed in triplicate. *P < 0.05, ** P < 0.01