Fig. 6

SNORA68 retains RPL23 in the nucleolus, leading to c-Myc expression via U2AF2. A Expression of c-Myc in SNORA68-overexpressing MDA-MB-231 and BT549 cells was verified by Western blot. B The binding site of RPL23 to U2AF2 was predicted by MOE software. C The interaction between endogenous RPL23 and U2AF2 was detected in SNORA68-overexpressing MDA-MB-231 cells by co-IP. D IF was performed to determine the colocalization of RPL23 in SNORA68-overexpressing MDA-MB-231 cells. Scale bars, 5 μm. E The expression of RPL23 in the nucleoplasm and nucleolus was measured by Western blot in different groups of TNBC cells. F Western blot was used to determine RPL23, U2AF2 and c-Myc expression in each group. The data are presented as the mean ± SD of three independent experiments performed in triplicate. **** P < 0.0001