Fig. 5

SNORA68 promotes the stemness of TNBC through U2AF2. A U2AF2 expression was detected by qRT-PCR in silencing U2AF2 of MDA-MB-231 and BT-549 cells. B U2AF2 expression was detected by Western blot in silencing U2AF2 of MDA-MB-231 and BT-549 cells. C The expression of stemness markers (Nanog, OCT4 and SOX2) in each group was verified by Western blot. D Self-renewal ability was detected in each group by sphere formation assays. Scale bars, 50 μm. E An in vitro LDA assay was used to determine the frequency of CSCs in each group. F, G The proliferation of TNBC cells with SNORA68 overexpression and U2AF2 knockdown was determined by CCK-8 and colony formation assays. H The migration of TNBC cells with SNORA68 overexpression and U2AF2 knockdown was determined by Transwell assay. Scale bars, 100 μm. The data are presented as the mean ± SD of three independent experiments performed in triplicate. * P < 0.05, ** P < 0.01, *** P < 0.001