Fig. 4

SNORA68 combines with U2AF2. A, B Bioinformatic analysis was used to predict the interaction between SNORA68 and U2AF2. C U2AF2 was detected by bioinformatic analysis in TCGA datasets. D IF and FISH were performed to determine the colocalization of SNORA68 and U2AF2 in MDA-MB-231 cells. Scale bars, 20 μm. E, F The binding sites of SNORA68 and U2AF2 were predicted by MOE software. G Silver staining of proteins enriched by SNORA68 compared with the negative control in MDA-MB-231 cells. H Western blot of U2AF2 expression in the SNORA68 pull-down precipitates in MDA-MB-231 cells. I qRT‒PCR analysis of SNORA68 levels in complexes enriched by RIP of U2AF2 in MDA-MB-231 and BT-549 cells. The data are presented as the mean ± SD of three independent experiments performed in triplicate. *P < 0.05, **P < 0.01