Fig. 3

SNORA68 enhances the stemness of TNBC cells. A SNORA68 expression was measured by qRT-PCR in SNORA68-overexpressing MDA-MB-231 and BT-549 cells. B SNORA68 expression in MDA-MB-231 and BT-549 cells in which SNORA68 was silenced was measured by qRT‒PCR. C The expression of stemness markers (Nanog, OCT4 and SOX2) in SNORA68-overexpressing MDA-MB-231 and BT549 cells was verified by Western blot. D The expression of stemness markers (Nanog, OCT4 and SOX2) in SNORA68-knockdown MDA-MB-231 and BT549 cells was verified by Western blot. E The self-renewal ability of SNORA68-overexpressing MDA-MB-231 and BT549 cells was detected by sphere formation assays. Scale bars, 50 μm. F Self-renewal ability was detected in SNORA68-knockdown MDA-MB-231 and BT549 cells by sphere formation assays. Scale bars, 50 μm. G, H An in vitro LDA assay was used to determine the CSC frequency in SNORA68-knockdown MDA-MB-231 and BT549 cells. The data are presented as the mean ± SD of three independent experiments performed in triplicate. * P < 0.05, ** P < 0.01