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Table 2 Cell cycle phase proportions in ER + BC cells with or without FGFR1 overexpression

From: Paradoxical cancer cell proliferation after FGFR inhibition through decreased p21 signaling in FGFR1-amplified breast cancer cells

Cell lines

Transfection

Sub G1%

G1%

S%

G2%

CAMA1

vector

1.7 ± 0.8

57.7 ± 2.0

22.1 ± 1.4

22.2 ± 1.6

 

FGFR1

1.0 ± 1.2

71.6 ± 2.5

18.4 ± 2.5

12.2 ± 1.4

MDA-MB-134

vector

0.7 ± 0.5

55.2 ± 0.5

31.2 ± 0.4

15.8 ± 1.2

 

FGFR1

0.5 ± 0.4

59 ± 1.6

31 ± 0.8

9.8 ± 1.1

MCF7

vector

1.4 ± 0.7

45.6 ± 1.0

39.6 ± 2.3

12.3 ± 4.8

 

FGFR1

1.0 ± 0.3

50 ± 1.0

40.5 ± 2.9

6.7 ± 3.5

T47D

vector

6.5 ± 1.3

61.7 ± 2.0

23.7 ± 1.9

6.5 ± 1.1

 

FGFR1

6.7 ± 0.6

69.8 ± 1.3

18.1 ± 1.3

6.2 ± 0.4

  1. The cells were transfected with FGFR1 or empty vectors and collected after 48 h, followed by PI staining-based flow cytometry analysis. The proportions of each phase were determined by Flowjo V10.3