Fig. 6
TET1 and TET3 bind to the UCHL1 gene promoter and contribute to promoter demethylation. A. The potential binding sites for TET1 and TET3 in the UCHL1 gene promoter regions. The analysis is based on data from the GSM2642522 and GSM1018960 datasets, which include ChIP-seq data using anti-TET1 or anti-TET3, respectively. B-E. The expression of TET1, TET3 and UCHL1 at both mRNA (B-C) and protein (D-E) levels in MD-MB-468 and SUM149 cells with TET1 or TET3 knockdown. F-G. Representative images (F) and quantitation (G) of BSP assays to detect the methylation of CpGs upon TET1 or TET3 knockdown in MDA-MB-468 and SUM149 cells. Data are represented as meanāĀ±āSD from three independent experiments. H. ChIP-qPCR assay was performed to show the enrichment of UCHL1 promoter segments in MDA-MB-468 and SUM149 cells with or without TET1 or TET3 knockdown. ChIP was conducted using anti-TET1 or anti-TET3, respectively. I.KLF5 mRNA (left) and protein (right) expression in MDA-MB-468 cells with TET1 or TET3 knockdown alone or in combination with UCHL1 overexpression. J. ESR1 mRNA expression in MDA-MB-468 and SUM149 cells with TET1 or TET3 knockdown. K. EGFR and ERĪ± protein expression in MDA-MB-468 cells with TET1 or TET3 knockdown. **, pā<ā0.01; ***, pā<ā0.001