Fig. 2

UCHL1 negatively regulates ERα expression and the responses to endocrine therapy via KLF5 in TNBC cells in vitro A-B. FGFBP1, EGFR, and ESR1 mRNA (A) and protein (B) expression in MDA-MB-468 and SUM149 cells with UCHL1 knockdown, KLF5 overexpression alone or in combination C-D. MDA-MB-468 and SUM149 cells with UCHL1 knockdown alone or combined with KLF5 overexpression were treated with Tamoxifen (C) or Fulvestrant (D) for 72 h. Cell viability was measured using CCK-8 assay. E. Colony formation of MDA-MB-468 and SUM149 cells with UCHL1 knockdown alone or in combination with KLF5 overexpression were treated with Tamoxifen (10 µM, top panels) or Fulvestrant (500 nM, bottom panels). Results were mean ± SD from three biologically independent experiments. ***, p < 0.001