Fig. 6

(A) Effects of p53 knockdown on DOX and OKI-005 sensitivity measured by CellTiter-Glo after 72 h drug exposure. (B) Bliss scores calculated from the data in Fig. 6A using Synergy Finder⁺. (C) Anti-proliferative effects of DOX (0.25 µM), OKI-005 (0.2 µM) or combination measured by live cell imaging for 48 h. (D) Pro-apoptotic effects measured by Annexin V/ PI after 48 h of DOX (0.5 µM), OKI-005 (0.2 µM), or combination. (E) Statistical analysis of apoptosis via total Annexin V. Ordinary one-way ANOVA with Tukey correction comparing single agent to combination (** = P < 0.01; *** = P < 0.001; **** = P < 0.001). (F) Effects of DOX and OKI-005 on downstream effectors of DNA damage, apoptosis, and cell cycle. Cells were treated for 24 h with DOX (0.5 µM), OKI-005 (0.2 µM), or combination. (G) Senescence-associated β-galactosidase staining of MDA-MB-231, Hs 578T, and CAL-120 after 6 days of drug treatment. DAPI staining portrays the nucleus. Representative images were taken at x20 magnification. Scale 50 μm