Table 2 Components of BTOM-ER growth media
| Â | Supplements/growth factors | Vendor | Catalog # | Final concentration |
|---|---|---|---|---|
Reagent A | ||||
1 | Bovine pituitary extract | Hammond cell tech | 1078-NZ | 0.8Â ml for 100Â ml |
2 | B27 | Thermo | 17504001 | 1.0Â ml for 100Â ml |
3 | Recombinant human FGF-basic (FGF2) | Peprotech | AF-100-18B | 10Â ng/ml |
4 | Recombinant human FGF10 | Peprotech | 100-26 | 10Â ng/ml |
5 | Recombinant human EGF | Peprotech | AF-100-15 | 2.0Â ng/ml |
6 | Recombinant human IL6 | Peprotech | 200-06 | 100Â ng/ml |
7 | Recombinant human amphiregulin | Peprotech | 100-55B | 100Â ng/ml |
8 | Recombinant human prolactin | Peprotech | 100-07 | 10Â ng/ml |
9 | Human insulin | Sigma | I2643-250MG | 10 μg/ml |
Reagent B | ||||
|  | Hydrocortisone | Sigma | 1316004-200MG | 0.5 μg/ml |