Fig. 2

PDXOs grown in BTOM maintain estrogen-dependent phenotypes of ER+ breast cancer. A Western blot showing Estrogen Receptor alpha expression in PDX011 and PDX5 organoids. VINCULIN was used as a loading control. B Luminescence levels in PDX011 and PDX5 organoids after 10 days of treatment with b-estradiol or vehicle control (EtOH). N = 3. C Representative brightfield images of Day 10 PDX011 and PDX5 grown in either BTOM or phenol red-free BTOM (left). Luminescence values for PDX011 and PDX5 organoids 10 days after being grown in either BTOM or phenol red-free BTOM (N = 3). D Luminescence values for PDX011 and PDX5 after being treated for 5 days with increasing doses of fulvestrant or vehicle control (N = 3)