Fig. 1

BTOM promotes robust growth and ER expression of ER+ PDXOs. A Schematic describing establishment of PDXOs. B A representative experiment of growth over time of PDX011 and PDX5 organoids, as measured by 3D Cell Titer Glo (Promega). Doubling time was calculated using GraphPad Prism Software. C H&E and brightfield images to show the morphology of established PDXOs. Scale bars: 100 μm. D Immunofluorescence of PDXOs displaying CK8/18 and ER-alpha expression. DAPI was used to stain nuclei