Fig. 6

3D single cell invasion and spheroid invasion in Collagen I ECM A Schematic depicting experimental set up of 3D invasion assay. B 3D invasion of breast cancer cell lines invading through Collagen I ECM C 3D persistence of cells invading through collagen I ECM D Representative rose plots of the invasion of cells through the collagen I ECM. E Schematic depiction of spheroid invasion assay. F Fold change in spheroid invasion into Collagen I hydrogels over invasion into media G Representative spheroid images of 231, BT549, Hs578T, and SUM159 spheroids cultured in media (upper) or spheroids cultured in Collagen I solution visualized with cytopainter (red). Scale bar = 500 μm. Data show mean ± SEM. Significance was determined using a one-way ANOVA with Tukey’s multiple comparison test comparing each cell line to every other cell line. Significance (p < 0.05) is denoted by a letter corresponding to each cell line tested, 231 (a), 468 (b), BT549 (c), BT20 (d), SUM159 (e), Hs578T (f)