Fig. 4

Determination of the significance of residues H343/L344 for the oncogenic roles of FAM83D in vivo. a-c, The empty vector control (CTRL), FAM83D WT-overexpressed (FAM83D WT), and FAM83D M2-overexpressed (FAM83D M2) MCF7 cells were subcutaneously inoculated in the nude mice (n = 5/group). Representative images of the dissected tumors. A ruler was used to demonstrate the size of the tumors (a). The tumor growth curve of each group was generated by measuring every 3 days (b). Quantification of tumor weights at the end point (c). d-e, The aforementioned cells were injected into the tail vein of nude mice (n = 5/group). Representative hematoxylin and eosin staining of metastatic foci per section in lung (d, upper) and liver (d, lower) of individual mouse. Quantification of the metastatic nodules per section in lung (e, left) and liver (e, right). f-h, FAM83D-silenced BT549 (shFAM83D) and their control cells (shNC) were subcutaneously inoculated in the nude mice (n = 4/group). Representative images of the dissected tumors. A ruler was used to demonstrate the size of the tumors (f). The tumor growth curve of each group was generated by measuring every 3 days (g). Quantification of tumor weights at the end point (h). i-j, The indicated BT-549 cells were injected into the tail vein of nude mice (n = 4/group). Representative hematoxylin and eosin staining of metastatic foci per section in lung (i, upper) and liver (i, lower) of individual mouse. Quantification of the metastatic nodules per section in lung (j, left) and liver (j, right). Data were presented as mean ± SD. ns: not significant. **: p < 0.01, ***: p < 0.001 based on the Student’s t-test