Fig. 1

Identification of the FBXW7-binding sites on FAM83D. a, Schematic diagram of wild type (WT FAM83D) protein structure and its truncations (R373, R350, F490, F365 and F330). b-c, Co-immunoprecipitation analysis of the interaction between FBXW7 and FAM83D truncation mutants: F490 and F330 (b), F365, R350 and R373 (c). d, Schematic diagram of WT FAM83D protein structure and the refined truncations (the residues 330 to 350). e, Determination of FAM83D fragments (F335 and F341) for FBXW7 binding through co-immunoprecipitation analysis. f, Sequence alignment of residues from the indicated 25 different species using UniProt according to the regions 341 to 450 of human FAM83D potentially involved in FBXW7 binding. g, Schematic diagram of FAM83D point mutants (M1-M3). h, Identification of the key residues for FBXW7 binding by co-immunoprecipitation analysis. The results were the representative of three independent experiments