Fig. 2

S1g-2 selectively disrupted Hsp70-Bim PPI, while MKT-077 preferentially disrupted Hsp70-Bag3 PPI. Western blot and co-IP analysis of the levels of Hsp70, Bim, Bag3, and their PPIs in MCF-7/TAM-R cells upon treatment with a gradient of concentrations (0–10 μM) of S1g-2 (A), MKT-077 (B), and VER-155008 (C) for 12 h, respectively, using β-actin as a loading control. An equivalent of DMSO were added to the compound untreated group as vehicle control. The graphs show (mean ± SD, n = 3 biologically independent experiments) Bim or Bag3 protein level in Hsp70 co-IP normalized against β-actin and relative to that in control. *P < 0.05, **P < 0.01 (one-way ANOVA test); n.s. indicates no significance between vehicle- and compounds-treated groups