Fig. 7

Glycolysis inhibition improves response to NAC in 3D TNBC cell models. A Colony-forming capacity of HCC38 and HCC1143 cells treated with 12.5 mM 2-DG alone or in combination with 0.04 nM and 0.12 nM paclitaxel respectively, for 3 days. B, C Growth of 3D spheroids initiated from HCC38 and HCC1937 cells treated with 50 mM 2-DG alone or in combination with 22 and 37.8 µM paclitaxel respectively B or 624 nM and 617.5 nM epirubicin respectively C for 3 days. D, E Growth of BCO17 and IDC031 TNBC organoids after treatment with 50 mM 2-DG alone or in combination with 50 nM paclitaxel D or 100 nM epirubicin E for 7 days. Data are plotted as the means ± SEM from n = 2–3 cultures, performed each time with ≥ 2 technical replicates (A–E). Significance was determined by two-way ANOVA with Tukey’s multiple comparison test (A–E). *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant