Fig. 3

Mitochondrial oxidative metabolism is decreased in TNBC cells upon treatment with chemotherapy. A–C Oxygen consumption rates (OCR) upon sequential treatment with 1 µM oligomycin, 2 µM FCCP and 0.5 µM rotenone/antimycin A (A), and OCR at basal (B) and maximal levels (C) in HCC1143 cells treated with 9 nM paclitaxel or 600 nM epirubicin for 24 h. D–I OCR values in HCC38 (D–F) and HCC1937 (G–I) in the same experimental conditions than indicated for HCC1143 cells (except that 4 and 300 nM paclitaxel as well as 25 and 800 nM epirubicin were used for HCC38 and HCC1937 cells, respectively). Data are plotted as the means ± SEM from n = 6 cultures, performed each time with ≥ 3 technical replicates (A–I). Significance was determined by one-way ANOVA (B, C, E, F, and H, I) with Tukey’s multiple comparison test. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant