Fig. 2

Expression of CD160-TM isoform by TNBC cell lines. a Total RNAs were extracted from TNBC (BT20, MDA-MB-453, IJG1731 and MDA-MB-453) and NK92 cell lines and subjected to reverse transcription. PCR were realized using a pair of primers corresponding to the 5′ and 3′ ends of CD160-GPI or CD160-TM reported coding sequence. Amplification of β-actin cDNA was performed in parallel as an internal control. b Analysis of CD160-GPI expression by TNBC cells. Cells were either left untreated (top panels) or subjected to a fixation/permeabilization step prior to labeling (bottom panels). Staining was performed using a PE-coupled anti-CD160-GPI mAb (BY55; grey histograms) or isotype control Igs (muIgM; white histograms). Cells were then analyzed by flow cytometry. c Analysis of CD160-TM expression by TNBC cells. Cells were labeled using APC-coupled anti-CD160-TM Ab (A12; grey histograms) or the corresponding isotype control (huIgG1; white histograms) and analyzed by flow cytometry