Fig. 6
Expression and distribution of extracellular matrix molecules in mammary glands of 30-month-old mice with different genotypes. (A) Representative immunofluorescent staining of laminin (LN), collagen I (ColI), and fibronectin (FN) in the mammary glands of mice with different genotypes (30-month-old Her2t/w/Adamts18+/+ and Her2t/w/Adamts18−/− mice). Blue, 4',6-diamidino-2-phenylindole (DAPI); white short arrow, fractured laminin; yellow short arrow, local accumulation of laminin; white arrow, Col I distributed in stroma of mammary glands; Green arrow, fibronectin distributed in BM of mammary glands. Scale bars = 50 μm. (B) Western blot analysis of the protein levels of LNα5 in mammary glands of mice (n = 3/group). (C-E) Levels of ColI (C), LN (D), and ColIV (E) measured by sandwich enzyme-linked immunosorbent assay (n = 6/group). Data are expressed as mean ± SD (n = 6). *p < 0.05; Student’s t-test, two tailed