Fig. 4

ADAMTS18 deficiency promotes mammary hyperplasia. (A) Whole-mount preparation of mammary glands from the indicated genotypes at 30 months of age. Scale bars = 500 μm. Arrowheads indicate branches and terminal end buds. LN, lymph node. (B) Quantification of the branch point in the two genotypes indicated in B (n = 3/group). (C) Representative H&E-stained cross-sections of mammary glands from the indicated genotypes. Arrowheads indicate thickened tubules. Scale bars = 100 μm. (D) Representative immunohistochemical staining of cross-sections of mammary glands from the indicated genotypes using Ki-67 antibody. Scale bars = 50 μm. (E) Quantification of Ki-67-positive cells (n = 4/group). Data are expressed as mean ± SD. *p < 0.05; ***p < 0.001; Student’s t-test, two tailed