Fig. 3

Increased proliferation, migration, and invasion capacity in Her2^t/w/Adamts18^−/− mammary tumor cells. (A) Counting kit-8 (CCK8) assay for cell proliferation of mammary tumor cells after 12-h (h) culture. (B–D) Migration of mammary tumors by scratch wound healing assays. Representative images of migrated mammary tumors in each group are shown (B). The scratch healing ratio was obtained by comparing the scratch area of 6 h and 12 h with that of 0 h. A smaller ratio indicates faster cell migration (healing) (C and D). Scale bar = 50 μm. (E–F) Transwell assay of invasive ability of mammary tumors. Representative images of migrated mammary tumors in each group are shown (E). Scale bar = 200 μm. Cells at the lower surface of the transwell chamber were counted (F). Data are expressed as mean ± SD from Her2^t/w/Adamts18^+/+ mammary tumors (n = 2), Her2^t/w/Adamts18^−/− mammary tumors (n = 3), and Her2^t/t mammary tumors (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant; one-way ANOVA