Fig. 1
Expression of Adamts18 mRNA in mouse mammary gland. (A) In situ hybridization analysis of Adamts18 mRNA in mammary gland of mice. In situ hybridization–positive signals appear as pink dots in cells. Scale bar = 50 μm. M, month. Neg, negative probe hybridization of mammary gland as negative control. (B) The expression of Adamts18 mRNA in mammary gland of mice aged from 1 to 30 months was analyzed by qRT-PCR. The relative quantity of Adamts18 mRNA was normalized to that of the housekeeping gene Gapdh using the ΔΔCt method. Each dot represents an individual. Data are expressed as mean ± SD