Fig. 5

USP5 promotes TNBC progression by regulating PFKP protein expression in vitro and in vivo. a The USP5 and PFKP protein expression in control and USP5 knockdown MDA-MB-231 cells in the presence or absence of overexpressed PFKP were analyzed by Western blot. b–f MDA-MB-231 cell proliferation following USP5 knockdown in the presence or absence of overexpressed PFKP was evaluated using CCK8 assay (b), Clone formation assay (c, d), and EDU incorporation assay (e, f). ***P < 0.001, **P < 0.01. g Representative images of the xenograft tumors in each group. h, i Tumor volume (h) and tumor weight (i) of nude mice in each group. **P < 0.01, *P < 0.05. j The PFKP protein expression in each sample of mice tumor tissue was analyzed by IHC staining. Scale bars, 100 μm