Fig. 2

Inhibition of PFKP reduces TNBC progression in vitro and in vivo. a, b qRT-PCR (a) and western blot (b) analysis of PFKP expression with PFKP knockdown in MDA-MB-231 cells. ***P < 0.001. c CCK-8 assay determination of MDA-MB-231 cell proliferation in response to PFKP knockdown. ***P < 0.001. d, e Clone formation assay determination of MDA-MB-231 cell proliferation in response to PFKP knockdown. ***P < 0.001, **P < 0.01. f, g EDU incorporation assay determination of MDA-MB-231 cell proliferation in response to PFKP knockdown. **P < 0.01. h Representative images of the xenograft tumors in each group. i, j PFKP knockdown reduces tumor volume (i) and tumor weight (j) of nude mice. ***P < 0.001. k The PFKP protein expression in each sample of mice tumor tissue was analyzed by IHC staining. Scale bars, 100 μm