Table 2 List of antibodies and protocols

CD105-AF488

SN6/IgG1

AbD Serotec/MCA1557A488

1:25

CD105

SN6

Abcam/Ab11414

1:100

M

CD26

202–36

Abcam/Ab3154

1:50

M

CD140b

PR7212

R&D Systems/MAB1263

1:200

F1

Tenascin

BC-24

Sigma, T2551

1:5000

M

Vimentin

SP20

ThermoFisher Scientific/RM-9120

1:200

FFPE

α-sm actin

1A4

Sigma/A2547

1:500–1:1000

1:1000

M

K19

A53b/a2

Abcam/ab7754

1:1000

M

CD31

89C2

Cell Signaling/3528S

1:1000

M

MYH11

SMMS-1

DAKO/M3558

1:50

M

CD146/MCAM

P1H12

Abcam/Ab24577

1:500

M

GATA3

HG3-31

Santa Cruz/sc-268

1:250

F2

ER

EP1

Dako/M3643

1:50

F2

PR

PgR636

Dako/M3569

1:100

F2

K5

XM26

Novocastra/NCL-L-CK5

1:250

M

K14

LL002

Monosan/MonX10687

1:100

M

HER2

TAB250

Invitrogen/28-0003Z

1:200

M

AF594

Anti-mouse

Invitrogen/A21203

1:500–1:1000

AF568

Anti-mouse IgG2a

Invitrogen/A21134

1:500