Fig. 2

Single-cell RNA sequencing reveals transcriptomic shifts in triple-negative breast cancer VMTs and VMOs. A Schematic showing workflow of scRNA-seq. Matched VMO and VMT were established for TNBC cell lines MDA-MB-231 and HCC1599. Cells were isolated from each model and submitted for scRNA-seq. B UMAP of VMO matched to MDA-MB-231 VMT shows endothelial cells (ECs), fibroblasts, cycling cells and stromal cells are present as C shown with the expression of key marker genes. D UMAP of MDA-MB-231 VMT shows ECs, fibroblasts, stromal cells, cycling cells and tumors are present as E demonstrated with the expression of key marker genes. F UMAP of VMO matched to HCC1599 shows ECs, fibroblasts, and stromal cells are present as G demonstrated with the expression of key marker genes. H UMAP of HCC1599 VMT shows ECs, fibroblasts, stromal cells, and tumors are present as I shown with the expression of key marker genes. J Integrated dataset containing all cells from the VMO (MDA-MB-231), VMO (HCC1599), MDA-MB-231 VMT and HCC1599 VMT. K Proportions of different cell types from each dataset. L Heatmap showing top 5 DEGs from each cell type from each dataset in the integrated space