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Fig. 6 | Breast Cancer Research

Fig. 6

From: Protein disulfide isomerase family member 4 promotes triple-negative breast cancer tumorigenesis and radiotherapy resistance through JNK pathway

Fig. 6

PDIA4 promoted tumorigenesis and RT resistance by binding and protecting TAX1BP1 against protein degradation. A PDIA4 can bind TAX1BP1 with a high binding fraction as determined by IP/MS analysis. B Co-IP experiments demonstrated that PDIA4 immunoprecipitated with anti-TAX1BP1 antibody, and TAX1BP1 immunoprecipitated with anti-PDIA4 antibody. C Immunofluorescence was conducted to examine the expression and localization of PDIA4 and TAX1BP1, the results showed that PDIA4 and TAX1BP1 was localized mainly in the cytoplasm of TNBC cells and cancer specimens. D Western blot analysis of TAX1BP1 expression levels in TNBC cells with PDIA4 knockdown or overexpression. E Western blot analysis of TAX1BP1 expression levels in TNBC cells treated with CHX for the indicated times. F MDA-MB-231 and MDA-MB-468 cells were treated with/without MG132, and TAX1BP1 protein levels were detected by western blot

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Breast Cancer Research

ISSN: 1465-542X

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