Fig. 4

PDIA4 inhibited apoptosis effects in TNBC cells with/without IR. A and B Flow cytometric analysis of apoptosis in MDA-MB-231 and MDA-MB-468 cells infected with lentivirus PDIA4 or shRNA3 with/without IR. C and D Western blot analysis showed that knockdown of PDIA4 increased the levels of cleaved-PARP and Bax in TNBC cells while reducing the expression level of Bcl-2 with/without IR and the opposite results were observed when PDIA4 was overexpression in TNBC cells with/without IR. E and F MMP was analyzed using immunofluorescence in TNBC cells of knockdown or overexpressing of PDIA4 with/without IR. G and H ROS was analyzed using immunofluorescence in TNBC cells of knockdown or overexpressing of PDIA4 with/without IR